Localization of bacterial DNA polymerase: Evidence for a factory model of replication
Localization of bacterial DNA polymerase: Evidence for a factory model of replication
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DOI：
10.1126/science.282.5393.1516
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发表时间：
1998-11-20
期刊：
影响因子：
56.9
通讯作者：
Grossman, AD
中科院分区：
文献类型：
--
作者：
Lemon, KP;Grossman, AD
已经提出了两种DNA复制的一般模型。在一个模型中，DNA聚合酶沿着DNA移动（就像轨道上的火车）;在另一个模型中，聚合酶是静止的（就像工厂），DNA被拉了过去。为了区分这些模型，我们通过创建包含催化亚基（PolC）和绿色荧光蛋白（GFP）的融合蛋白来可视化活细胞中细菌枯草芽孢杆菌的DNA聚合酶。PolC-GFP定位于离散的细胞内位置，主要在中细胞处或附近，而不是随机分布。这些结果表明，聚合酶被锚定在适当的位置，从而支持DNA模板移动通过聚合酶的模型。
Two general models have been proposed for DNA replication. In one model, DNA polymerase moves along the DNA (Like a train on a track); in the other model, the polymerase is stationary (Like a factory), and DNA is pulled through. To distinguish between these models, we visualized DNA polymerase of the bacterium Bacillus subtilis in living cells by the creation of a fusion protein containing the catalytic subunit (PolC) and green fluorescent protein (GFP). PolC-GFP was Localized at discrete intracellular positions, predominantly at or near midcell, rather than being distributed randomly. These results suggest that the polymerase is anchored in place and thus support the model in which the DNA template moves through the polymerase.