Analysis of fungal endophytes in Scottish Sitka spruce plantations shows extensive infections, novel host partners and gives insights into origins
Analysis of fungal endophytes in Scottish Sitka spruce plantations shows extensive infections, novel host partners and gives insights into origins
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DOI：
10.1111/efp.12471
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发表时间：
2018-10
期刊：
影响因子：
1.4
通讯作者：
Carly R. Anderson Stewart;M. Doilom;Joanne E. Taylor
中科院分区：
文献类型：
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作者：
Carly R. Anderson Stewart;M. Doilom;Joanne E. Taylor
本初步研究鉴定了苏格兰四个人工林中锡特卡云杉（Picea sitchensis）的真菌内生菌，分析了它们在不同地点的招募可能性和群落结构，并确定了这种非本地寄主内生菌的可能来源。尽管锡特卡云杉原产于北美西北部，但它在苏格兰森林中占有很大一部分，是一种经济上至关重要的木材。收集两个年龄级的针叶，培养新出现的内生菌分离株。利用代表性形态型的形态特征和核糖体ITS区域的基因测序相结合，记录了57种形态型，代表了至少15个不同的物种，其中包括北美和欧洲假性门氏假糖（pseudosuga menziesiiiii）病原体的假设姐妹种，Nothophaeocryptopus gaeumannii(先前为p。Gaeumannii)在所有测试地点都存在。讨论了多样性、招募、地点、针龄和针部效应。通过对其ITS和Tub2序列数据的综合分析，对该内生菌的系统发育进行了研究，以确定该内生菌与花旗松病原菌n的关系。gaeumannii。
This preliminary study identified the fungal endophytes of Sitka spruce (Picea sitchensis)in four Scottish plantations, analysed their recruitment possibilities and community structure across the sites, and identified the possible origins of the endophytes in this non‐native host. Although Sitka spruce is native to north‐western North America, it comprises a huge portion of Scottish forestry and is an economically vital timber tree. Needles from two age classes were collected and cultured for emerging endophyte isolates. Using a combination of morphological features and genetic sequencing of the nuclear ribosomal ITS regions of representative morphological types, 57 morphotypes were recorded which represented at least 15 different species, including a hypothesized sister species to the pathogen ofPseudotsuga menziesiiin North America and Europe,Nothophaeocryptopus gaeumannii(previouslyP. gaeumannii) that was present at all sites tested. Diversity, recruitment, site, needle age and needle part effects are discussed. The phylogeny ofNothophaeocryptopusbased on analysis of combined ITS and Tub2 sequence data is given in order to assess the relationship of the unknown endophyte to the Douglas fir pathogenN. gaeumannii.