Longitudinal 1H MRS assessment of the thalamus in a Coriaria lactone‐induced rhesus monkey status epilepticus model
Longitudinal 1H MRS assessment of the thalamus in a Coriaria lactone‐induced rhesus monkey status epilepticus model
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DOI：
10.1002/nbm.2789
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发表时间：
2012-10
影响因子：
2.9
通讯作者：
Xiao-Yun Zhang;Zhiyong Yang;Jinmei Li;Hong-xia Li;Li Wang;Q. Gong;Dong Zhou
中科院分区：
文献类型：
--
作者：
Xiao-Yun Zhang;Zhiyong Yang;Jinmei Li;Hong-xia Li;Li Wang;Q. Gong;Dong Zhou
神经生理学、生物化学和解剖学证据表明丘脑在癫痫发作中发挥作用。然而，直到最近，纵向表征在体内丘脑动力学还没有报道。在这项研究中，我们研究了丘脑的代谢，以确定马桑内酯（CL）诱导癫痫持续状态（SE）后发生的变化，并观察癫痫样放电是否可以在左右丘脑之间存在差异。5只恒河猴在配有12通道头部线圈的Siemens Trio Tim 3-T MR扫描仪上接受了全脑MRI和单体素MRS。使用LCModel处理光谱。在SE前和SE发作后1、7、21和42天对5只动物进行扫描。对所获得的数据进行统计分析，结果表明健康猕猴的双侧丘脑无显著差异。我们的MRS数据显示SE后N-乙酰天冬氨酸在左右丘脑中对称分布（p = 0.003）。此外，该纵向研究表明SE后1天双侧丘脑中谷氨酸/谷氨酰胺升高（p < 0.05）和肌醇降低（p < 0.05），所有代谢物在第5次扫描时接近其基线水平。我们的研究结果表明，在CL诱导的恒河猴SE期间，丘脑发生代谢变化。各种代谢变化可能表明左侧丘脑更容易受到癫痫发作的影响。版权所有© 2012约翰威利父子有限公司.
Neurophysiological, biochemical and anatomical evidence implicates the thalamus as playing a role in epileptic seizures. Until recently, however, longitudinal characterization of in vivo thalamus dynamics had not been reported. In this study, we investigated the metabolism in the thalamus to identify the changes that occur following Coriaria lactone (CL)‐induced status epilepticus (SE) and to observe whether the epileptiform discharges could present a difference between the left and right thalami. Five rhesus monkeys underwent whole‐brain MRI and single‐voxel MRS on a Siemens Trio Tim 3‐T MR scanner with a 12‐channel head coil. Spectra were processed using LCModel. Scans were performed in five animals before SE and at 1, 7, 21 and 42 days after the onset of SE. Statistical analysis of the data obtained demonstrated no significant difference in the bilateral thalamus of healthy macaques. Our MRS data showed symmetrical distributions of N‐acetylaspartate in the right and left thalami after SE (p = 0.003). In addition, this longitudinal study demonstrated elevated glutamate/glutamine (p < 0.05) and reduced myo‐inositol (p < 0.05) in the bilateral thalamus 1 day after SE, and all metabolites approached their baseline levels by the fifth scan. Our results demonstrate that metabolic changes occur in the thalamus during CL‐induced SE in rhesus monkeys. The various metabolic changes may indicate that the left thalamus is more vulnerable to epileptic strike. Copyright © 2012 John Wiley & Sons, Ltd.