Membrane and cytoplasmic nitrate reductase of Staphylococcus aureus and application of crossed immunoelectrophoresis.
Membrane and cytoplasmic nitrate reductase of Staphylococcus aureus and application of crossed immunoelectrophoresis.
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金黄色葡萄球菌膜和胞质硝酸盐还原酶及交叉免疫电泳的应用。
DOI：
10.1128/jb.148.2.724-727.1981
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发表时间：
1981
影响因子：
3.2
通讯作者：
Lascelles,J
中科院分区：
文献类型：
--
作者：
Burke,KA;Brown,AE;Lascelles,J
金黄色葡萄球菌膜硝酸还原酶的特异性抗血清源自交叉免疫电泳板上的免疫沉淀。对用硝酸盐和叠氮化物生长的细胞中酶的细胞质和膜形式的分析表明了它们的同一性，并且在每种情况下，主要亚基 Mr 140,000 被胰蛋白酶转化为多肽 Mr 112,000，而没有损失酶活性或免疫反应性。
Specific antiserum to the membrane nitrate reductase of Staphylococcus aureus was derived from immunoprecipitates on crossed immunoelectrophoresis plates. Analysis of the cytoplasmic and membrane forms of the enzyme in cells grown with nitrate and azide indicated their identity, and in each case, the major subunit, Mr 140,000, was converted by trypsin to a polypeptide, Mr 112,000, without loss of enzyme activity or immunological reactivity.