Resistance to DNA fragmentation and chromatin condensation in mice lacking the DNA fragmentation factor 45.
Resistance to DNA fragmentation and chromatin condensation in mice lacking the DNA fragmentation factor 45.
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DOI：
10.1073/pnas.95.21.12480
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发表时间：
1998-10
影响因子：
11.1
通讯作者：
Jianhua Zhang;Xuesong Liu;David C. Scherer;L. Kaer;Xiaodong Wang;Ming Xu
中科院分区：
文献类型：
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作者：
Jianhua Zhang;Xuesong Liu;David C. Scherer;L. Kaer;Xiaodong Wang;Ming Xu
DNA 断裂因子 45 (DFF45) 是异二聚体核酸酶复合物的一个亚基，对于体外诱导 DNA 断裂至关重要。为了了解 DFF45 在程序性细胞死亡中的体内作用，我们培育了 DFF45 突变小鼠。 DFF45 突变组织的细胞提取物中 DNA 片段化活性完全消失。为了响应细胞凋亡刺激，DFF45 突变小鼠的脾细胞、胸腺细胞和粒细胞能够抵抗 DNA 断裂，并且脾细胞和胸腺细胞也能够抵抗染色质浓缩。尽管如此，DFF45 突变小鼠的免疫系统发育是正常的。这些结果表明，DFF45 对于体内 DNA 片段化和染色质浓缩的诱导至关重要，但对于正常免疫系统的发育不是必需的。
The DNA fragmentation factor 45 (DFF45) is a subunit of a heterodimeric nuclease complex critical for the induction of DNA fragmentation in vitro. To understand the in vivo role of DFF45 in programmed cell death, we generated DFF45 mutant mice. DNA fragmentation activity is completely abolished in cell extracts from DFF45 mutant tissues. In response to apoptotic stimuli, splenocytes, thymocytes, and granulocytes from DFF45 mutant mice are resistant to DNA fragmentation, and splenocytes and thymocytes are also resistant to chromatin condensation. Nevertheless, development of the immune system in the DFF45 mutant mice is normal. These results demonstrate that DFF45 is critical for the induction of DNA fragmentation and chromatin condensation in vivo, but is not required for normal immune system development.