A Progestin-Based Treatment with a High Dose of Estradiol Benzoate Normalizes Cyclic Changes in Endometrial EGF Concentrations and Restores Fertility in Repeat Breeder Cows
A Progestin-Based Treatment with a High Dose of Estradiol Benzoate Normalizes Cyclic Changes in Endometrial EGF Concentrations and Restores Fertility in Repeat Breeder Cows
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DOI：
10.1262/jrd.20078
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发表时间：
2008-12-01
影响因子：
1.8
通讯作者：
Takahashi, Yoshiyuki
中科院分区：
文献类型：
--
作者：
Katagiri, Seiji;Takahashi, Yoshiyuki
本研究的目的是检查以孕激素为基础的治疗与高剂量的苯甲酸雌二醇（EB），以正常化的表皮生长因子（EGF）在子宫内膜和恢复生育力的重复育种奶牛的疗效。在整个研究中使用了在发情周期的第3天和第14天子宫内膜EGF浓度没有峰值的重复育种奶牛。首先检查了1（标准剂量）、2.5和5 mg EB在基于孕激素的治疗方案（分别为EB 1、EB 2.5和EB 5治疗;每组n=5）中对子宫内膜EGF浓度的影响。与生育对照组（n = 5）中的EB 1治疗相比，在重复繁殖母牛中的EB 1和EB 2.5治疗产生了子宫内膜EGF的抑制反应，并且在下一个发情周期中未能恢复正常的EGF特征。然而，EB 5治疗产生了类似于生育对照的EGF浓度的增加，并使子宫内膜EGF谱正常化。EB 4和EB 5处理（每组n=30）对子宫内膜EGF分布和生育力的影响然后在重复繁殖母牛中检查。EB 5组（66.7%）中EGF谱经治疗标准化的奶牛比例高于EB 1组（30.0%）和未治疗对照组（13.3%; n=30）（P < 0.01）。EB 1和EB 5组治疗后EGF谱正常的奶牛的妊娠率相似（分别为88.9%和85.0%），高于同组内EGF谱全部异常的奶牛的妊娠率（分别为19.0%和30.0%，P < 0.01）。总之，在重复繁殖母牛中，就EGF浓度而言，子宫内膜对EB的反应受到抑制。在第3天和第14天子宫内膜EGF浓度较低的重复繁殖母牛中，发现在基于孕激素的治疗中高剂量（5 mg）EB可有效恢复正常EGF特征和生育力。
The objective of the present study was to examine the efficacy of a progestin-based treatment with a high dose of estradiol benzoate (EB) to normalize the epidermal growth factor (EGF) profile in the uterine endometrium and restore fertility in repeat breeder cows. Repeat breeder cows without peaks in their endometrial EGF concentrations on Days 3 and 14 of the estrous cycle were used throughout the study. The effect of I (standard dose), 2.5 and 5 mg of EB ill a progestin-based treatment protocol (EB1, EB2.5 and EB5 treatments, respectively; n=5 for each group) oil endometrial EGF concentrations was first examined. The EB1 and EB2.5 treatments in the repeat breeder cows produced a Suppressed response in endometrial EGF compared with EB1 treatment in the fertile controls (n = 5) and failed to restore the normal EGF profile during the next estrous cycle. However, EB5 treatment produced an increase in EGF concentrations similar to the fertile controls and normalized the endometrial EGF profile. The effects of the EB4 and EB5 treatments (n=30 for each group) oil the endometrial EGF profile and fertility were then examined in the repeat breeder cows. The proportion of cows, with an EGF profile normalized by the treatments was higher in the EB5 group (66.7%) than in the EB1 (30.0%,) and untreated control (13.3%,; n=30) groups (P < 0.01.). The pregnancy rates of the cows having, a normal EGF profile after treatment in the EB1 and EB5 groups were similar (88.9 and 85.0%, respectively) and hi-her than those of the cows having all abnormal profile within the same groups (19.0 and 30.0%,, respectively, P < 0.01). In summary, the endometrial response to EB in terms of the EGF concentration Was Suppressed in repeat breeder cows. A high dose (5 mg) of EB in a progestin-based treatment was found to be effective for restoration of a normal EGF profile and fertility in repeat breeder cows having lesser endometrial EGF concentrations oil Days 3 and 14.