Synchronization of cellular clocks in the suprachiasmatic nucleus
Synchronization of cellular clocks in the suprachiasmatic nucleus
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DOI：
10.1126/science.1089287
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发表时间：
2003-11-21
期刊：
影响因子：
56.9
通讯作者：
Okamura, H
中科院分区：
文献类型：
--
作者：
Yamaguchi, S;Isejima, H;Okamura, H
作为昼夜节律中心的视交叉上核(SCN)中的单个细胞时钟被整合到一个稳定而强健的起搏器中，其周期长度约为24小时。在器官型切片培养中，我们使用实时基因表达分析来显示哺乳动物SCN内数百个神经元中时钟基因转录的同步节奏。不同相位的神经元时钟在SCN上呈地形性排列。蛋白质合成抑制剂将所有细胞时钟设置在相同的初始阶段，在退出后，细胞时钟之间的内在相互作用重新建立了跨组装的基因表达的稳定程序。Na+依赖的动作电位有助于建立细胞同步性和维持跨SCN的自发振荡。
Individual cellular clocks in the suprachiasmatic nucleus (SCN), the circadian center, are integrated into a stable and robust pacemaker with a period length of about 24 hours. We used real-time analysis of gene expression to show synchronized rhythms of clock gene transcription across hundreds of neurons within the mammalian SCN in organotypic slice culture. Differentially phased neuronal clocks are topographically arranged across the SCN. A protein synthesis inhibitor set all cell clocks to the same initial phase and, after withdrawal, intrinsic interactions among cell clocks reestablished the stable program of gene expression across the assemblage. Na+-dependent action potentials contributed to establishing cellular synchrony and maintaining spontaneous oscillation across the SCN.