Novel reverse radioisotope labelling experiment reveals carbon assimilation of marine calcifiers under ocean acidification conditions
Novel reverse radioisotope labelling experiment reveals carbon assimilation of marine calcifiers under ocean acidification conditions
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新型反向放射性同位素标记实验揭示了海洋酸化条件下海洋钙化物的碳同化
DOI：
10.1111/2041-210x.13396
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发表时间：
2020
影响因子：
6.6
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
8.Nishida;K.;Chew;Y. C.;Miyairi;Y.;Hirabayashi;S.;Suzuki;A.;Hayashi;M.; Yamamoto;Y.;Sato;M.;Nojiri;Y.;Yokoyama ;Y.
人为二氧化碳排放导致的海洋酸化预计会抑制海洋钙化物的代谢和生理活动。为了评估海洋生物对海洋酸化的敏感性，必须检查碳酸盐壳和软组织中营养物质的同化情况。我们设计了一种新颖的实验方案，即反向放射性同位素标记，以追踪海洋酸化条件下单个双壳类物种内营养物质的分配。注入不含放射性碳的二氧化碳气体，可以在水中溶解的碳与从大气中吸收的碳之间形成巨大的对比。通过在酸化海水中培养现代含水层生物，我们能够确定终端成员、海水中溶解的无机碳 (DIC) 和代谢二氧化碳对碳酸盐壳和软组织的相对贡献的差异。在所有 pCO2 条件下（463、653、872、1,137 和 1,337 µatm），放射性碳 双壳类Scapharca Bringoniishell的(Δ14C)值与海水DIC值显着相关；因此，贝壳碳酸盐主要来源于海水 DIC。 Δ14C 结果与稳定碳同位素 (δ13C) 数据一起表明 inS。 Bringoniishell δ13C 可能反映同位素平衡动力学以及端元贡献；因此，在通过先前使用 δ13C 的方法分析端元贡献时必须小心。 S 的不敏感性。 pCO2 中的扰动至少达到 1,337 µatm 表明该物种可以承受海洋酸化。使用放射性同位素进行示踪实验需要严格的规则来进行任何操作。然而，本研究提出的反向放射性同位素标记具有很大的优势，是了解含水层生物生理学的有力工具，可适用于各种生物和培养实验，如温度、盐度和酸化实验，以提高对海洋生物在变化环境下吸收营养物质比例的了解。
Ocean acidification by anthropogenic carbon dioxide emissions is projected to depress metabolic and physiological activity in marine calcifiers. To evaluate the sensitivity of marine organisms against ocean acidification, the assimilation of nutrients into carbonate shells and soft tissues must be examined.We designed a novel experimental protocol, reverse radioisotope labelling, to trace partitioning of nutrients within a single bivalve species under ocean acidification conditions. Injecting CO2gas, free from radiocarbon, can provide a large contrast between carbon dissolved in the water and the one assimilated from atmosphere. By culturing modern aquifer organisms in acidified seawater, we were able to determine differences in the relative contributions of the end members, dissolved inorganic carbon (DIC) in seawater and metabolic CO2, to shell carbonate and soft tissues.Under allpCO2conditions (463, 653, 872, 1,137 and 1,337 μatm), radiocarbon (Δ14C) values of the bivalveScapharca broughtoniishell were significantly correlated with seawater DIC values; therefore, shell carbonate was derived principally from seawater DIC. The Δ14C results together with stable carbon isotope (δ13C) data suggest that inS. broughtoniishell δ13C may reflect the kinetics of isotopic equilibration as well as end‐member contributions; thus, care must be taken when analysing end‐member contributions by a previous method using δ13C. The insensitivity ofS. broughtoniito perturbations inpCO2up to at least 1,337 µatm indicates that this species can withstand ocean acidification.Usage of radioisotope to dope for tracer experiments requires strict rules to conduct any operations. Yet, reverse radioisotope labelling proposing in this study has a large advantage and is a powerful tool to understanding physiology of aquifer organisms that can be applicable to various organisms and culture experiments, such as temperature, salinity and acidification experiments, to improve understanding of the proportions of nutrients taken in by marine organisms under changing environments.