Expression of pepper cyclophilin gene is differentially regulated during the pathogen infection and abiotic stress conditions
Expression of pepper cyclophilin gene is differentially regulated during the pathogen infection and abiotic stress conditions
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DOI：
10.1006/pmpp.2001.0356
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发表时间：
2001-10-01
影响因子：
2.7
通讯作者：
Hwang, BK
中科院分区：
文献类型：
--
作者：
Kong, HY;Lee, SC;Hwang, BK
从辣椒黄单胞菌致病变种（Xanthomonascampestrispv.）vesicatoria使用差异杂交技术。CACYP 1基因推导的氨基酸序列与番茄、马铃薯、豆类和拟南芥亲环素基因的氨基酸序列具有高度的同源性。CACYP 1 mRNA在辣椒植株的叶、茎、根和花中组成型表达，但在绿色未成熟果实和红色（成熟）果实中不表达。辣椒叶片中CACYP 1 mRNA的积累受X.野油菜致病变种vesicatoria和胶孢炭疽菌Colletotrichum gloeosporioide。感染.与相容性相互作用相比，在不相容性相互作用中，接种细菌的叶片中CACYP 1 mRNA发生了剧烈的诱导。接种X.野油菜致病变种vesicatoria在下部叶片中导致在未接种的上部叶片中强烈的系统性CACYP 1 mRNA积累。原位杂交结果表明，CACYP 1 mRNA定位于辣椒果实维管组织的韧皮部细胞中。胶孢菌水杨酸和茉莉酸甲酯处理强烈诱导辣椒叶片中CACYP 1的转录。(C)北京：科学出版社.
A pathogen induced pepper cyclophilin (CACYP1) cDNA clone was isolated from a pepper cDNA library from hypersensitive response (HR) lesions of leaves infected with Xanthomonas campestris pv. vesicatoria using a differential hybridization technique. The deduced amino acid sequences OF this CACYP1 gene have high levels of, identity with those Of cyclophilin genes from tomatoes, potatoes, beans and Arabidopsi.. The CACYP1 mRNA was constitutively expressed in the leaf, stem, root and flower, but not in the green unripe: and the red (ripe) fruits of pepper plants. The accumulation of CACYP1 mRNA was strongly induced in pepper plants by X. campestris pv. vesicatoria and Colletotrichum gloeosporioide. infections. In the incompatible interaction, a drastic induction of the CACYP1 mRNA occurred in the bacteria inoculated leaves compared to that of the compatible interaction. Prior inoculation with an avirulent strain of X. campestris pv. vesicatoria in lower leaves resulted in a strong, systemic CACYP1 mRNA accumulation in the uninoculated upper leaves. A situ hybridization results revealed that CACYP1 mRNA was localized in the phloem cells of vascular tissues in pepper fruits infected by C. gloeosporioides. Treatment with salicyclic acid and methyl jasmonate strongly induced the CACYP1 transcripts in pepper leaves. (C) 2001 Academic Press.