HGPRT- mutants of V79 cells that revert specifically by base pair substitution and frameshift mutations.
HGPRT- mutants of V79 cells that revert specifically by base pair substitution and frameshift mutations.
复制标题
DOI：
10.1002/em.2860070305
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
D. Stone-Wolff;C. Klein;T. Rossman
中科院分区：
文献类型：
--
作者：
D. Stone-Wolff;C. Klein;T. Rossman
为了确定哺乳动物细胞中诱变剂和致癌剂的诱变特异性，能够区分移码突变和各种碱基对取代的回复系统将是有用的。我们在此报告了一种 HGPRT-中国仓鼠 V79 细胞突变体的分离和表征方法，该方法可能构成此类系统的基础。两种不同特异性的突变体已被部分表征。 DEW-1 在 N-甲基-N'-硝基-N-亚硝基胍 (MNNG) 处理后分离，可通过碱基对取代诱变剂 MNNG 和甲磺酸乙酯 (EMS) 恢复，但不能通过移码诱变剂恢复。 ICR-191 处理后分离出的 DSW-3 会被移码诱变剂特异性逆转，但 EMS 或 MNNG 则不会。随着这些和其他突变体的进一步表征，不仅可以确定某种试剂在 V79 细胞中是否具有诱变性，而且还可以确定其产生的突变类型。
In order to determine the mutagenic specificity of mutagenic and carcinogenic agents in mammalian cells, a reversion system capable of distinguishing between frameshift mutations and various kinds of base pair substitutions would be useful. We report here a method for the isolation and characterization of HGPRT- Chinese hamster V79 cell mutants that might form the basis for such a system. Two mutants of different specificity have been partially characterized. DEW-1, isolated following N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) treatment, is revertible by the base pair substitution mutagens MNNG and ethyl methanesulfonate (EMS), but not by frameshift mutagens. DSW-3, isolated following ICR-191 treatment, is specifically reverted by frameshift mutagens, but not by EMS or MNNG. With the further characterization of these and other mutants, it should be feasible to determine not only whether an agent is mutagenic in V79 cells, but also to determine the type(s) of mutation(s) it produces.