Comparative evaluation of adhesion and biofilm formation of different Listeria monocytogenes strains
Comparative evaluation of adhesion and biofilm formation of different Listeria monocytogenes strains
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DOI：
10.1016/s0168-1605(00)00406-2
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发表时间：
2000-12-05
影响因子：
5.4
通讯作者：
Schraft, H
中科院分区：
文献类型：
--
作者：
Chae, MS;Schraft, H
13种单核细胞增生李斯特菌菌株用于在37 ℃的静态条件下在玻璃表面上生长生物膜长达4天。在最初的3小时粘附后和随后的1天间隔中，在从玻璃表面擦拭细胞后使用标准平板计数来确定细胞数。采用激光共聚焦扫描显微镜（CSLM）观察了原位生物膜的三维结构。培养3 h后，所有13株L.发现单核细胞增生菌附着在玻璃载玻片上，并且所有菌株在24小时内形成生物膜。不同菌株在生物膜上的粘附能力差异显著，24 h后生物膜上的细胞数差异显著。在4天内监测单核细胞增生菌株。头2天计数增加，达到10（5）cfu/cm（2），除了L。单核细胞增多症7148（10（4）cfu/cm（2））。2天后，4个菌株的细胞计数保持在10（5）cfu/cm（2）（在第3天和第4天测试），而L.单核细胞增多症7148继续生长并在第4天达到10（5）cfu/cm（2）。生物膜生长的这种差异与增殖细胞生长速率的变化无关，这表明生物膜中李斯特菌的生长行为可能与其增殖生长不同。CSLM显示，在静态条件下生长的生物膜由两个不同的层组成，与上层相比，底层的细胞数高0.5 log（10）。(C)2000 Elsevier Science B. V.保留所有权利。
Thirteen Listeria monocytogenes strains were used to grow biofilms on glass surfaces in static conditions at 37 degreesC for up to 4 days. After the initial 3-h adhesion and in subsequent 1-day intervals, cell numbers were determined using standard plate count after swabbing the cells from the glass surface. The three-dimensional structure of in situ biofilms was determined by confocal scanning laser microscopy (CSLM). After 3 h incubation, bacterial cells for all 13 strains of L. monocytogenes were found attached to glass slides and all strains formed biofilms within 24 h. The strains varied significantly in their ability to adhere to the surface and significant differences for cell numbers after 24 h biofilm growth were found. Cell counts in biofilms formed by five L. monocytogenes strains were monitored over 4 days. The counts increased for the first 2 days reaching 10(5) cfu/cm(2), except for L. monocytogenes 7148 (10(4) cfu/cm(2)). After 2 days, cell counts remained at 10(5) cfu/cm(2) for four strains (tested on days 3 and 4), while L. monocytogenes 7148 continued to grow and reached 10(5) cfu/cm(2) on day 4. This difference in biofilm growth was not related to variations in growth rates of planktonic cells suggesting that growth behaviour of Listeria in biofilms may be different from their planktonic growth. CSLM revealed that the biofilms grown under static conditions consisted of two distinct layers with 0.5 log(10) higher cell numbers in the bottom layer as compared to the upper layer. (C) 2000 Elsevier Science B.V. All rights reserved.