Changes of substance P during fracture healing in ovariectomized mice
Changes of substance P during fracture healing in ovariectomized mice
复制标题
DOI：
10.1016/j.regpep.2009.11.004
复制
发表时间：
2010-01-08
影响因子：
--
通讯作者：
Dai, Li-Yang
中科院分区：
文献类型：
--
作者：
Ding, Wen-Ge;Zhang, Zi-Ming;Dai, Li-Yang
神经肽在骨折愈合过程中可能起重要作用。为探讨P物质在骨质疏松性骨折愈合过程中的作用，将192只小鼠随机分为卵巢切除组（OVX）和对照组（CON），每组96只。OVX后3周造成股骨干骨折。分别于骨折后1、2、4、8周测定骨密度（BMD）、骨痂形成和矿化的micro-CT（mu CT）分析、骨折部位新生血管和生物力学性能的mu CT分析以及P物质水平，并与CON组进行比较。骨折端P物质含量在各时间点均显著降低，且早期（1、2周）高于晚期（4、8周）。在OVX小鼠中也观察到血管生成受损。血清P物质水平在不同时间点和不同组间均无显著性差异。结论：骨质疏松性骨折愈合较差，P物质水平显著降低，P物质可能在骨质疏松性骨折愈合过程中起重要作用。(C)2009爱思唯尔有限公司版权所有。
Neuropeptides may play an important role in the healing process of osteoporotic fractures. The objective of this study was to determine the role of substance P during osteoporotic fracture healing.One hundred ninety-two mice were randomized into ovariectomy (OVX) and control (CON) group (n = 96, respectively). Femoral shaft fracture was created 3 weeks after OVX. Bone mineral density (BMD), micro-CT (mu CT) analysis of fracture callus formation and mineralization, mu CT analysis of fracture site neovascularization and biomechanical property as well as substance P levels were evaluated 1, 2, 4, and 8 weeks after fracture and compared with CON group.Following OVX-induced bone loss, fracture healing in OVX mice was significantly poorer than that in CON mice, with a significant decrease of substance P at the fracture site at all time points and with the level at early stage (1 and 2 weeks) higher than later stage (4 and 8 weeks). impaired angiogenesis was also noted in OVX mice. No significant change of substance P level in serum was found between different groups or time points.In conclusion, fracture healing is inferior in OVX-induced bone loss and associated with a significant decrease of substance P. Substance P may play an important role during osteoporotic fracture healing. (C) 2009 Elsevier B.V. All rights reserved.