Constitutive Expresser of Pathogenesis Related Genes 1 Is Required for Pavement Cell Morphogenesis in Arabidopsis.
Constitutive Expresser of Pathogenesis Related Genes 1 Is Required for Pavement Cell Morphogenesis in Arabidopsis.
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拟南芥路面细胞形态发生需要发病机制相关基因1的组成型表达
DOI：
10.1371/journal.pone.0133249
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Hou S
中科院分区：
文献类型：
--
作者：
Han B;Chen L;Wang J;Wu Z;Yan L;Hou S
50多年来，研究人员一直关注细胞骨架在控制细胞生长方向和细胞扩增中的重要作用。在本研究中，我们以Col-0为背景进行了甲烷磺酸乙酯诱变，并鉴定了两个新的与路面细胞（PC）形态发生缺陷有关的发病相关基因1 （CPR1）组成表达子等位基因。形态学表征表明，在cpr1中，pc的极性生长起始和扩展受到严重抑制。进一步的细胞骨架研究表明，cpr1突变体在PC发育过程中微管（MTs）的定向排列存在缺陷，皮质细肌动蛋白丝不能有效聚集形成肌动蛋白电缆网络。这些结果表明，cpr1中PC形态发生异常是伴随着细胞骨架排列异常而发生的。F-box-associated （FBA）结构域是识别CPR1特定底物的基序，其定点突变和敲除证明FBA结构域对于正常的CPR1对PC形态发生的调控是不可或缺的。进一步的遗传分析表明，cpr1的PC形态发生缺陷依赖于两种脂酶样蛋白，即疾病易感性增强1和PHYTOALEXIN缺陷4。我们的研究结果进一步揭示了细胞骨架与PC形态发生之间的关系，并表明细胞骨架介导的PC形态发生控制可能与植物的防御反应密切相关。
For over 50 years, researchers have focused on the mechanisms underlying the important roles of the cytoskeleton in controlling the cell growth direction and cell expansion. In our study, we performed ethyl methane sulfonate mutagenesis on Col-0 background and identified two new CONSTITUTIVE EXPRESSER OF PATHOGENESIS RELATED GENES 1 (CPR1) alleles with pavement cell (PC) morphogenetic defects. Morphological characterizations showed that polar growth initiation and expansion of PCs are seriously suppressed in cpr1. Closer cytoskeleton investigation showed that the directional arrangement of microtubules (MTs) during PC development is defective and the cortical fine actin filaments cannot be aggregated effectively to form actin cable networks in cpr1 mutants. These results suggest that the abnormal PC morphogenesis in cpr1 is accompanying with the aberrant arrangement of cytoskeleton. Site-directed mutagenesis and knockout within the F-box-associated (FBA) domain, which is reported to be a motif for recognizing particular substrates of CPR1, proved that the FBA domain is indispensable for normal CPR1 regulation of the PC morphogenesis. Further genetic analysis indicated that the defects on PC morphogenesis of cpr1 depend on two lipase-like proteins, ENHANCED DISEASE SUSCEPTIBILITY 1 and PHYTOALEXIN DEFICIENT 4. Our results provide further insights into the relationship between the cytoskeleton and PC morphogenesis, and suggest that the cytoskeleton-mediated PC morphogenesis control might be tightly linked to plant defense responses.