Specific knockdown of the D2 long dopamine receptor variant.
Specific knockdown of the D2 long dopamine receptor variant.
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DOI：
10.1097/wnr.0b013e32834d2216
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发表时间：
2012-01-04
期刊：
影响因子：
1.7
通讯作者：
Gu HH
中科院分区：
文献类型：
--
作者：
Naughton BJ;Thirtamara-Rajamani K;Wang C;During MJ;Gu HH
多巴胺信号传导在介导可卡因的作用中是至关重要的。多巴胺D2受体有两种剪接变体，D2L和D2S，它们被认为具有不同的功能作用。在这里，我们表明，使用病毒介导的shRNA选择性地敲低D2L导致基础运动活性轻微但显著的降低，而可卡因诱导的运动刺激没有显著变化。击倒似乎产生了减少可卡因的条件性位置偏好的趋势，但差异没有统计学意义。我们的研究结果表明，D2受体的剪接变体可以在体内特定的脑区进行选择性操作，从而可以对每个D2受体亚型进行更具体的研究。
Dopamine signaling in the nucleus accumbens is critical in mediating the effects of cocaine. There are two splice variants of dopamine D2 receptors, D2L and D2S, which are believed to have different functional roles. Here we show that knocking down D2L selectively using viral mediated shRNA led to a slight but significant decrease in basal locomotor activity with no significant change in cocaine induced stimulation of locomotion. The knockdown appears to produce a trend of reduced conditioned place preference to cocaine but the difference was not statistically significant. Our results demonstrated that the splice variants of D2 receptors can be selectively manipulated in vivo in specific brain regions allowing more specific studies of each D2 receptor isoform.