Expression patterns in alternative splicing forms of prosaposin mRNA in the rat facial nerve nucleus after facial nerve transection
Expression patterns in alternative splicing forms of prosaposin mRNA in the rat facial nerve nucleus after facial nerve transection
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DOI：
10.1016/j.neures.2007.09.010
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发表时间：
2008-01-01
影响因子：
2.9
通讯作者：
Matsuda, Seiji
中科院分区：
文献类型：
--
作者：
Chen, Jie;Saito, Shouichiro;Matsuda, Seiji
Prosaposin 除了作为 saposin A、B、C 和 D 的前体蛋白外，还充当神经营养因子，saposin A、B、C 和 D 是溶酶体中特定鞘脂水解酶的激活剂。在大鼠中，prosaposin 基因产生两种选择性剪接形式的 mRNA：含有 9 个碱基插入的 Pro + 9 和不含 9 个碱基插入的 Pro + 0。这些 mRNA 的表达在脑损伤后发生变化。我们在面神经横断后 52 天的时间内检查了大鼠面神经核中 prosaposin mRNA 的选择性剪接形式的表达模式。 Pro+0 mRNA在横切后3天内增加，5-10天后达到峰值，并在21天内保持显着升高。相反，Pro+9 mRNA 的表达在整个再生期间保持恒定。在面神经再生过程中，Prosaposin mRNA 表达不仅在面部运动神经元中增加，而且在小胶质细胞中也增加。我们的研究结果表明，prosaposin 的 saposin B 结构域是受选择性剪接影响的结构域，在神经再生过程中在神经元和小胶质细胞中发挥着重要作用。 (c) 2007 Elsevier Ireland Ltd 和日本神经科学学会。版权所有。
Prosaposin acts as a neurotrophic factor, in addition to its role as the precursor protein for saposins A, B, C, and D, which are activators for specific sphingolipid hydrolases in lysosomes. In rats, the prosaposin gene generates two alternative splicing forms of mRNA: Pro + 9 containing a 9-base insertion and Pro + 0 without. The expression of these mRNAs changes after brain injury. We examined the expression patterns of the alternative splicing forms of prosaposin mRNA in the rat facial nerve nucleus for 52 days following facial nerve transection. Pro + 0 mRNA increased within 3 days of transection, peaked after 5-10 days, and remained significantly elevated for 21 days. In contrast, the expression of Pro + 9 mRNA was constant throughout the regenerative period. Prosaposin mRNA expression increased not only in facial motoneurons, but also in microglia during facial nerve regeneration. Our findings indicate that the saposin B domain of prosaposin, which is the domain affected by alternative splicing, plays an important role in both neurons and microglia during neuroregeneration. (c) 2007 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.