Assessing PfGARP-Mediated Apoptosis of Blood-Stage Plasmodium falciparum Parasites.
Assessing PfGARP-Mediated Apoptosis of Blood-Stage Plasmodium falciparum Parasites.
复制标题
评估 PfGARP 介导的血期恶性疟原虫寄生虫的细胞凋亡。
DOI：
10.1007/978-1-0716-2189-9_49
复制
发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Kurtis,Jonathan
中科院分区：
文献类型：
--
作者：
Mohapatra,AlokDas;Zuromski,Jenna;Kurtis,Jonathan
细胞凋亡通常被认为是一个进化上保守的和遗传控制的程序性细胞死亡过程，仅限于后生动物生物。然而，最近，在单细胞真核生物中也发现了凋亡的保守特征（Holzmuller等Parasitology 132：S19-S32，2006; Le Chat等Mol Biochem Parasitol 153：41-47，2007; Madeo等Curr Opin Microbiol 7：655-660，2004; Welburn等Parasitology 132：S7-S18，2006;詹森等Science 216：1230-1233，1982），包括疟疾寄生虫（Al-Olayan等人Int J Parasitol 32：1133-1143，2002; Ch’ng等人Cell Death Dis 1：e26，2010; Meslin等人J Infect Dis 195：1852-1859，2007; Picot等人Trans R Soc Trop Med Hyg 91：590-591，1997; Raj等人Nature 582：104-108，2020）J. falciparumglutamic-acid-rich protein（PfGARP）是一种80 kDa的抗原，其独特地表达在由早期至晚期滋养体阶段P感染的红细胞（RBC）的外表面上。恶性疟原虫（Raj等人，Nature 582：104-108，2020）。我们最近已经证明，PfGARP的抗体结合PfGARP的表面上展示的P。恶性疟原虫滋养体感染的RBC并触发细胞内寄生虫的细胞凋亡（Raj等，Nature 582：104-108，2020）。这是血液期疟疾寄生虫中抗体诱导的细胞凋亡的首次证明，其特征在于几个保守特征，例如危机形态、线粒体膜电位丧失、食物液泡完整性丧失、半胱氨酸蛋白酶样半胱氨酸蛋白酶的激活和染色体DNA的片段化。在这里，我们描述了用于检测这些功能的细胞凋亡的疟原虫成熟的血液阶段的测定。
Apoptosis is conventionally regarded as an evolutionarily conserved and genetically controlled process of programmed cell death confined to metazoan organisms. However, recently, conserved features of apoptosis have also been demonstrated in unicellular eukaryotes (Holzmuller et al. Parasitology 132:S19–S32, 2006; Le Chat et al. Mol Biochem Parasitol 153:41–47, 2007; Madeo et al. Curr Opin Microbiol 7:655–660, 2004; Welburn et al. Parasitology 132:S7–S18, 2006; Jensen et al. Science 216:1230–1233, 1982) including malaria parasites (Al-Olayan et al. Int J Parasitol 32:1133–1143, 2002; Ch’ng et al. Cell Death Dis 1:e26, 2010; Meslin et al. J Infect Dis 195:1852–1859, 2007; Picot et al. Trans R Soc Trop Med Hyg 91:590–591, 1997; Raj et al. Nature 582:104–108, 2020).P. falciparumglutamic-acid-rich protein (PfGARP) is an antigen of 80 kDa that is uniquely expressed on the exofacial surface of red blood cells (RBCs) infected by early-to-late-trophozoite-stageP. falciparumparasites (Raj et al. Nature 582:104–108, 2020). We have recently demonstrated that antibodies against PfGARP bind to the PfGARP displayed on the surface ofP. falciparumtrophozoite-infected RBCs and trigger apoptosis in the intracellular parasites (Raj et al. Nature 582:104–108, 2020). This is the first demonstration of antibody-induced apoptosis in blood-stage malaria parasites and is characterized by several conserved features such as crisis form morphology, loss of mitochondrial membrane potential, loss of integrity of food vacuole, activation of caspase-like cysteine proteases, and fragmentation of chromosomal DNA. Here we describe the assays used to detect these features of apoptosis in the mature blood stage of malaria parasites.