Chimeric pheromone receptors in the basidiomycete Schizophyllum commune
Chimeric pheromone receptors in the basidiomycete Schizophyllum commune
复制标题
DOI：
10.1006/fgbi.2000.1222
复制
发表时间：
2000-08-01
影响因子：
3
通讯作者：
Kothe, E
中科院分区：
文献类型：
--
作者：
Gola, S;Hegner, J;Kothe, E
担子菌裂褶菌的信息素受体系统能够识别配体，从而赋予交配特异性。信息素受体的B α基因座的配体歧视的结构域交换实验的策略进行了研究。已经发现了几种改变的嵌合受体表型，其中包括组成型信息素受体，它不需要配体来激活下游级联反应。此外，受体仍然依赖于配体被确定，已改变信息素激活配置文件，包括混杂受体，激活的信息素的所有9个特异性，包括前自我。此外，还产生了高度区分性的受体，这些受体仅被八种非自身特异性中的两种激活。嵌合受体将受体的最后三分之一鉴定为B α 1特异性的决定因素，而B α 2特异性存在于覆盖受体分子的第一和中间部分的非连续结构域中。(C)北京大学出版社.
The pheromone receptor system of the basidiomycete Schizophyllum commune is capable of ligand discrimination to confer mating specificity. The pheromone receptors of the B alpha locus were investigated for ligand discrimination in a strategy of domain swapping experiments. Several altered phenotypes of chimeric receptors have been found. These include constitutive pheromone receptors which need no ligand for activation of the downstream cascade of events. In addition, receptors still dependent on ligand were identified that had altered pheromone activation profiles, including promiscuous receptors that are activated by pheromones of all nine specificities, including the former self. In addition, highly discriminative receptors were created which are activated by only two of the eight non-self-specificities. The chimeric receptors identify the last third of the receptor as the determinant for B alpha1 specificity, whereas B alpha2 specificity resides in noncontiguous domains covering the first and middle parts of the receptor molecule. (C) 2000 Academic Press.