Differential gene expression in incompatible interaction between turnip mosaic virus and non-heading Chinese cabbage
Differential gene expression in incompatible interaction between turnip mosaic virus and non-heading Chinese cabbage
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DOI：
10.1007/s10658-011-9885-0
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发表时间：
2012-03
影响因子：
1.8
通讯作者：
Hai Peng;Li Wang;Y. Li;Yanxiao Li;W. Guan;Yang Yang-Yang;Xiaohui Xu;X. Hou
中科院分区：
文献类型：
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作者：
Hai Peng;Li Wang;Y. Li;Yanxiao Li;W. Guan;Yang Yang-Yang;Xiaohui Xu;X. Hou
由芜菁花叶病毒（Turnipmosaicvirus，TuMV）引起的病毒病是不结球白菜（Brassicacampestrisssp.chinensisMakino）的主要病害。虽然TuMV抗性基因已在甘蓝类蔬菜中定位，但TuMV与不结球白菜的分子互作机制尚不清楚。此外，需要鉴定TuMV反应基因。本研究旨在筛选TuMV与不结球白菜不亲和互作过程中差异表达的基因，并验证其表达。共200个转录衍生片段（TDFs）互补DNA扩增片段长度多态性获得回收和测序。结果表明，176个（88.0%）TDFs产生特异性序列，其中48个（27.3%）序列经NCBI BLAST预测具有推测的功能。在48个可用的TDFs中，选择了22个（45.8%）属于不同功能组的序列，通过实时定量聚合酶链反应监测它们在不相容和相容相互作用中的表达变化。据我们所知，本研究提供了第一个全球的不结球白菜基因在不亲和互作的转录组学分析。这些结果有助于了解TuMV应答基因的特征，阐明TuMV与宿主相互作用的分子机制。
Viral disease, caused by turnip mosaic virus (TuMV), is considered the most destructive disease of non-heading Chinese cabbage (Brassica campestrisssp.chinensisMakino). Although several TuMV resistance loci/genes have been mapped or characterized inBrassicavegetables, the mechanism of molecular interaction between TuMV and non-heading Chinese cabbage is poorly understood. Additionally, TuMV response genes need to be identified. The objectives of this study were to identify differentially expressed genes during the incompatible interaction between TuMV and non-heading Chinese cabbage, and validate their expressions. A total of 200 transcript-derived fragments (TDFs) obtained by complementary DNA-amplified fragment length polymorphism were recovered and sequenced. The results revealed that 176 (88.0%) TDFs produced specific sequences, among which 48 (27.3%) sequences were predicted with putative functions using NCBI BLAST. Among the 48 available TDFs, 22 (45.8%) sequences belonging to different functional groups were selected to monitor the changes in their expression in incompatible and compatible interactions by quantitative real-time polymerase chain reaction. To the best of our knowledge, this study provides the first global transcriptomic analysis of non-heading Chinese cabbage genes during an incompatible interaction. The results are expected to aid in characterizing TuMV response genes and clarifying the molecular mechanism of TuMV–host interaction.