ROLE OF ASPHYXIA AND FEEDING IN A NEONATAL RAT MODEL OF NECROTIZING ENTEROCOLITIS
ROLE OF ASPHYXIA AND FEEDING IN A NEONATAL RAT MODEL OF NECROTIZING ENTEROCOLITIS
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DOI：
10.3109/15513819409037698
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发表时间：
1994-11-01
期刊：
影响因子：
--
通讯作者：
HSUEH, W
中科院分区：
文献类型：
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作者：
CAPLAN, MS;HEDLUND, E;HSUEH, W
坏死性小肠结肠炎（NEC）是一种常见的胃肠道疾病，影响早产儿。 为了严格调查NEC（配方奶粉喂养，窒息，细菌和早产）的危险因素的重要性，我们开发了一种密切模仿人类疾病的新生大鼠模型。 对足月和早产新生大鼠进行配方喂养、窒息和/或外源性细菌定植应激，随后对肠道损伤的发展进行大体和组织学评价。 我们发现，大多数窒息、配方奶粉喂养和细菌处理的动物在96小时内发生NEC（77%）并死亡（86%）。 所有接受窒息和细菌定植处理的母体喂养动物均存活，且肠道组织学正常。 此外，窒息是一个关键的诱发因素，因为配方奶粉和细菌暴露没有窒息导致正常的肠道和最低的死亡率（12%）。 在该模型中，肠道细菌定植不是NEC的重要决定因素。 结论：新生大鼠模型是研究NEC的理想实验系统。 与人类疾病一样，窒息和配方奶粉喂养在实验性NEC的病理生理学中起重要作用。
Necrotizing enterocolitis (NEC) is a common gastrointestinal disorder affecting premature infants. To investigate critically the importance of the purported risk factors of NEC (formula feeding, asphyxia, bacteria, and prematurity), we developed a neonatal rat model that closely mimics the human disease. Full-term and premature newborn rats were stressed with formula feeding, asphyxia, and/or exogenous bacterial colonization and subsequently evaluated grossly and histologically for the development of intestinal injury. We found that most animals treated with asphyxia, formula feeding, and bacteria developed NEC (77%) and died (86%) by 96 h. All maternally fed animals treated with asphyxia and bacterial colonization survived and had normal intestinal histology. Furthermore, asphyxia was a critical instigating factor, because formula and bacterial exposure without asphyxia resulted in normal intestine and minimal mortality (12%). Enteral bacterial colonization was not a significant determinant of NEC in this model. We conclude that the neonatal rat model is an excellent test system for the study of NEC. As in the human disease, asphyxia and formula feeding play an important role in the pathophysiology of experimental NEC.