Maximizing specificity and yield of PCR by the quantum dot itself rather than property of the quantum dot surface
Maximizing specificity and yield of PCR by the quantum dot itself rather than property of the quantum dot surface
复制标题
通过量子点本身而不是量子点表面的特性最大化 PCR 的特异性和产量
DOI：
10.1016/j.biochi.2009.04.020
复制
发表时间：
2009-08-01
期刊：
影响因子：
3.9
通讯作者：
Li, Lijia
中科院分区：
文献类型：
--
作者：
Ma, Lu;He, Shibin;Li, Lijia
We found that semiconductor quantum dots (QDs) dramatically improved both product yield and specificity of PCR. The concentration of QDs is important for improving PCR amplification. In the presence of appropriate concentration of mercaptoacetic acid (MAA)-coated QDs, specificity and yield of PCR were enhanced. Also, strong nonspecific bands and weaker smeared bands were eliminated. At lower annealing temperatures (25-45 degrees C), addition of MAA-coated QDs into the PCR reagent produced specific PCR products without nonspecific sequence amplification. MAA alone did not improve PCR amplification. Streptavidin (SA) surface modified QDs with different size also effectively improved the specificity of PCR, demonstrating that the observed effect was not due to property of the QD surface but instead due to the QD itself. Bovine Serum Albumin (BSA) could relieve Taq polymerase from MAA-coated QDs in PCR by interaction with QDs and therefore imply that QDs improve specificity of PCR by interaction with Taq polymerase. These results demonstrate that QDs, added to reaction mixes at appropriate concentrations, can increase PCR yield and improve PCR specificity, even at low annealing temperatures. We assume that many different surface modified polymeric nanoparticles might have similar effects. (C) 2009 Elsevier Masson SAS. All rights reserved.
