Didehydrogeranylgeranyl (Delta Delta GG): a fluorescent probe for protein prenylation.
Didehydrogeranylgeranyl (Delta Delta GG): a fluorescent probe for protein prenylation.
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二脱氢香叶基香叶基 (Delta Delta GG)：蛋白质异戊二烯化的荧光探针。
DOI：
10.1021/ja0119144
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发表时间：
2002
影响因子：
15
通讯作者：
Prestwich,GlennD
中科院分区：
文献类型：
--
作者：
Liu,Xiao-hui;Prestwich,GlennD
The first intrinsically fluorescent analog of geranylgeraniol, (2E,6E,8E,10E,12E,14E)-geranylgeraniol (all-trans-ΔΔGGOH·1) has been synthesized stereoselectively and shown to substitute for the geranylgeranyl (GG) moiety in prenyl transferase reactions and in protein−ligand binding assays. All-trans-ΔΔGGOH1showed blue fluorescence in methanol, with λex= 310 nm and λem= 410 nm (ε310= 2.4 × 104M-1cm-1), but was only weakly fluorescent in aqueous solution. The prenyl transferase efficiency for ΔΔGGPP2as a substrate for yeast protein geranylgeranyl transferase (PGGTase-I) was 60% relative to that for GGPP. The binding of ΔΔGG-AcCysMe3to the recombinant Rho GTPase dissociation inhibitor (RhoGDI) had aKDof 15.1 ± 1.2 μM, 6-fold lower than the affinity of GG-AcCysMe. Thus, the ΔΔGG moiety is a novel fluorophore suitable for studying the interaction and subcellular localization of prenylated small GTPase proteins in signaling complexes.
