Genetic analysis of culture-negative UNAIDS subtype C samples
Genetic analysis of culture-negative UNAIDS subtype C samples
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DOI：
10.1089/08892220360473961
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发表时间：
2003-01-01
影响因子：
1.5
通讯作者：
López-Galíndez, C
中科院分区：
文献类型：
--
作者：
Sánchez-Merino, V;Herrero, CC;López-Galíndez, C
为了帮助开发疫苗，世卫组织-联合国艾滋病规划署启动了一项分离和鉴定C亚型病毒的计划，C亚型病毒是世界上最流行的HIV-1亚型。分离株来自巴西、中国、印度、以色列和南非，这些国家是这些菌株流行的国家。在这项研究中，我们的基因特征的一组样品显示培养阴性表型的核苷酸序列的三个基因组区域：p17区域的gag基因，C2 V3 C3片段的env基因，和nef基因。研究了nef基因与培养阴性表型的相关性，我们发现基因改变的显著积累。除了一个来自印度的B/C重组体，研究的样品形成了一个单系发生亚型C进化枝，虽然来自巴西的样品在分析的三个基因中的两个中形成了统计学显著的独立亚群。
To help in the vaccine development, WHO-UNAIDS launched a program for the isolation and characterization of subtype C viruses, the most prevalent HIV-1 subtype in the world. Isolates were obtained from Brazil, China, India, Israel, and South Africa, countries in which these strains are circulating. In this study we genetically characterized a set of samples displaying the culture-negative phenotype by sequencing the nucleotides of three genomic regions: the p17 region of the gag gene, the C2V3C3 fragment of the env gene, and the nef gene. The association of the culture-negative phenotype with the nef gene was studied, and we found a significant accumulation of gene alterations. Except for one B/C recombinant from India, the samples studied formed amonophylogepetic subtype C clade, although samples from Brazil formed a statistically significant, independent subcluster in two of the three genes analyzed.