Ontogeny and innate properties of neonatal dendritic cells
Ontogeny and innate properties of neonatal dendritic cells
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DOI：
10.1182/blood-2002-09-2966
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发表时间：
2003-07-15
期刊：
影响因子：
20.3
通讯作者：
Lo-Man, R
中科院分区：
文献类型：
--
作者：
Sun, CM;Fiette, L;Lo-Man, R
我们研究了树突状细胞（DC）区室发育不成熟是否会导致新生儿对感染的高易感性。 DC 是最早定植于脾脏的细胞之一，但 DC 亚群的个体发育遵循不同的步骤。出生时，脾脏中存在浆细胞样 DC 和 CD4(-)CD8α(-) DC，而 CD8α(+) 和 CD4(+) DC 不存在。然后，CD8α(+) DC 区室快速发育并在第 7 天达到成人大小，而 CD4+ DC 区室缓慢增大，到 3 周龄时成为主要细胞。 DCs 产生白细胞介素 (IL)-12p70 在出生后特别有效，反映出新生儿 CD8α(-) DCs 分泌 IL-12 的能力比成人对应物更强。同样，新生儿 DC 产生 I 型和 II 型干扰素。在体内，微生物刺激后，诱导了主要组织相容性复合物 (MHC) 和 DC 上共刺激分子的上调，清楚地显示了新生儿环境中新生儿 DC 的激活。因此，尽管生命早期的 DC 子集组成与成人 DC 区室相比明显不同，但新生 DC 完全具有先天免疫功能。 (C) 2003 年，美国血液学会。
We investigated whether a developmental immaturity of the dendritic cells (DCs) compartment could contribute to the high susceptibility to infections observed in newborns. DCs are among the first cells to colonize the spleen, but the ontogeny of DC subsets follows distinct steps. At birth, plasmacytoid DCs and CD4(-)CD8alpha(-) DCs are found in the spleen, whereas CD8alpha(+) and CD4(+) DCs are not present. Then, the CD8alpha(+) DC compartment quickly develops and reaches an adult size by day 7, whereas the CD4+ DC compartment slowly increases to become predominant by the age of 3 weeks. The production of interleukin (IL)-12p70 by DCs is particularly efficient after birth, reflecting the stronger capacity of the neonatal CD8alpha(-) DCs to secrete IL-12 compared with its adult counterpart. Likewise, neonatal DCs produced type I and II interferons. In vivo, following microbial stimulation, up-regulation of major histocompatibility complexes (MHCs) and of costimulatory molecules on DCs was induced clearly showing the activation of neonatal DCs in the neonatal environment. Therefore, despite a markedly different DC subset composition in early life compared with the adult DC compartment, neonatal DCs are fully competent in their innate immune functions. (C) 2003 by The American Society of Hematology.