Phylogenetic Patterns of Codon Evolution in the ACTIN-DEPOLYMERIZING FACTOR/COFILIN (ADF/CFL) Gene Family.
Phylogenetic Patterns of Codon Evolution in the ACTIN-DEPOLYMERIZING FACTOR/COFILIN (ADF/CFL) Gene Family.
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DOI：
10.1371/journal.pone.0145917
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Meagher RB
中科院分区：
文献类型：
--
作者：
Roy-Zokan EM;Dyer KA;Meagher RB
肌动蛋白解聚因子/cofilin （ADF/CFL）基因家族编码多种相对较小的蛋白质。一旦被严格地称为肌动蛋白丝动力学的调节剂，最近的研究表明，这些蛋白质参与多种细胞过程，从信号转导到肌动蛋白的细胞核运输。在植物和动物谱系中，ADF/CFL基因家族中类似物的表达模式因组织类型和发育阶段而异。在这项研究中，我们使用计算方法来研究导致ADF/CFL基因家族多样化的进化力量。估计跨系统发育谱系的非同义到同义突变率（dN/dS）显示，大多数ADF/CFL密码子位置处于强烈的纯化选择下，罕见的加速蛋白质进化事件。在植物和动物中，这些加速进化的实例都是ADF/CFL亚类特异性的，并且所有被选择的位点都位于可以发挥新功能作用的蛋白质区域。我们认为这些位点可能在ADF/CFL蛋白的功能多样化中起重要作用。
The actin-depolymerizing factor/cofilin (ADF/CFL) gene family encodes a diverse group of relatively small proteins. Once known strictly as modulators of actin filament dynamics, recent research has demonstrated that these proteins are involved in a variety of cellular processes, from signal transduction to the cytonuclear trafficking of actin. In both plant and animal lineages, expression patterns of paralogs in the ADF/CFL gene family vary among tissue types and developmental stages. In this study we use computational approaches to investigate the evolutionary forces responsible for the diversification of the ADF/CFL gene family. Estimating the rate of non-synonymous to synonymous mutations (dN/dS) across phylogenetic lineages revealed that the majority of ADF/CFL codon positions were under strong purifying selection, with rare episodic events of accelerated protein evolution. In both plants and animals these instances of accelerated evolution were ADF/CFL subclass specific, and all of the sites under selection were located in regions of the protein that could serve in new functional roles. We suggest these sites may have been important in the functional diversification of ADF/CFL proteins.