Anthelmintic Activity of Curcuma Aeruginosa Roxb Extract on Fasciola gigantica in Vitro
Anthelmintic Activity of Curcuma Aeruginosa Roxb Extract on Fasciola gigantica in Vitro
复制标题
铜绿姜黄提取物对巨片形吸虫的体外驱虫活性
DOI：
10.1051/e3sconf/202015101046
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发表时间：
2020
影响因子：
--
通讯作者：
Farida Athaillah
中科院分区：
文献类型：
--
作者：
H. Vanda;Rizki Parindra;M. Hambal;Farida Athaillah
片形吸虫是一种寄生虫，它会破坏脂肪、蛋白质和碳水化合物的代谢，从而干扰生长并导致死亡。姜黄提取物是一种药用植物，已被用于治疗多种疾病。本研究的目的是确定C. aeruginosa Roxb对F.包括死亡时间和治疗后发生的组织病理学变化。本研究采用完全随机设计，重复5次。将吸虫浸泡在三种不同的浸提液浓度中：10%（T1）、25%（T2）和50%（T3）（w/v）。磷酸盐缓冲盐水（PBS）溶液用作阴性对照（C1），阿苯达唑用作阳性对照（C2）。F.计算各组中大片吸虫的数量，并制备死亡吸虫进行组织病理学研究。资料以变异数分析进行统计分析。结果表明，C.浓度为10、25和50%的铜绿提取物分别在75、57和48分钟内导致吸虫死亡。组织学观察表明，浸提液引起了呼吸和营养吸收的重要器官--皮层的破坏。本研究认为C.铜绿假单胞菌提取物对F.试管中的甘草。
Fasciola gigantica is a parasite that causes a disruption of the metabolism of fats, proteins, and carbohydrates, which interferes growth and causes death. Curcuma aeruginosa Roxb extract is one of the medicinal plants which has been used to treat several diseases. The aim of this study was to determine the effect of methanolic extract of C. aeruginosa Roxb on F. gigantica, including mortality time and histopathological changes that occurred after treatment. This study used a completely randomized design with five replications. The flukes were soaked in three different extract concentrations: 10% (T1), 25% (T2), and 50% (T3) (w/v). Phosphate buffered saline (PBS) solution was used as a negative control (C1) and albendazole as the positive control (C2). The mortality time of F. gigantica in each group was calculated, and the dead flukes were prepared for histopathological study. The data were analyzed by Analysis of variance. The results showed that C. aeruginosa extracts at the concentration of 10, 25 and 50% caused the death of the flukes within 75, 57 and 48 minutes, respectively., Histopathological observations showed that the extract caused breakage of tegument which is an important organ in the respiratory process and nutrient absorption. This study concluded that C. aeruginosa extract exhibited anthelmintic activity towards F. gigantica in vitro.