D(-)-Salicin inhibits the LPS-induced inflammation in RAW264.7 cells and mouse models
D(-)-Salicin inhibits the LPS-induced inflammation in RAW264.7 cells and mouse models
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D(-)-Salicin 抑制 RAW264.7 细胞和小鼠模型中 LPS 诱导的炎症
DOI：
10.1016/j.intimp.2015.04.016
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发表时间：
2015-06-01
影响因子：
5.6
通讯作者：
Feng, Haihua
中科院分区：
文献类型：
--
作者：
Li, Yang;Wu, Qianchao;Feng, Haihua
D(-)-水杨素是一种传统药物，已被认为具有抗炎和其他治疗活性。本研究旨在探讨D(-)-水杨素在体内和体外对脂多糖诱导的炎症反应的抑制作用。采用酶联免疫吸附试验和免疫印迹法检测D(-)-水杨素对体内外细胞因子(TNF-α、IL-1β、IL-6和IL-10)的影响以及体内信号转导通路(MAPKs和NF-kappa B)的影响。结果表明，D(-)-水杨素可显著降低肿瘤坏死因子-α、IL-1β和IL-6的浓度，升高IL-10的浓度。此外，Western印迹分析表明，D(-)-水杨素可抑制脂多糖刺激的MAPKs和NF-kappaB信号通路的激活。为探讨D(-)-水杨素是否能减轻脂多糖诱导的肺部炎症反应，给小鼠腹腔注射MAPKs和核因子-kappaB信号通路的抑制剂。特异性抑制剂的干扰显示，D(-)-水杨素介导的细胞因子抑制是通过MAPKs和NF-kappa B途径实现的。在小鼠急性肺损伤模型上，组织病理学检查显示D(-)-水杨素能抑制内毒素所致的肺水肿。提示D(-)-水杨素可能是一种潜在的抗炎症性疾病的药物。(C)2015爱思唯尔B.V.保留所有权利。
D(-)-Salicin is a traditional medicine which has been known to exhibit anti-inflammation and other therapeutic activities. The present study aimed to investigate whether D(-)-Salicin inhibited the LPS-induced inflammation in vivo and in vitro. We evaluated the effect of D(-)-Salicin on cytokines (TNF-alpha, IL-1 beta, IL-6 and IL-10) in vivo and in vitro by enzyme-linked immunosorbent assay and signaling pathways (MAPKs and NF-kappa B) in vivo by Western blot. The results showed that D(-)-Salicin markedly decreased TNF-alpha, IL-1 beta and IL-6 concentrations and increased IL-10 concentration. In addition, western blot analysis indicated that D(-)-Salicin suppressed the activation of MAPKs and NF-kappa B signaling pathways stimulated by LPS. To examine whether D(-)-Salicin ameliorated LPS-induced lung inflammation, inhibitors of MAPKs and NF-kappa B signaling pathways were administrated intraperitoneally to mice. Interference with specific inhibitors revealed that D(-)-Salicin-mediated cytokine suppression was through MAPKs and NF-kappa B pathways. In the mouse model of acute lung injury, histopathologic examination indicted that D(-)-Salicin suppressed edema induced by LPS. So it is suggest that D(-)-Salicin might be a potential therapeutic agent against inflammatory diseases. (C) 2015 Elsevier B.V. All rights reserved.