Next generation sequence assembly with AMOS.
Next generation sequence assembly with AMOS.
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下一代序列组件与AMOS。
DOI：
10.1002/0471250953.bi1108s33
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发表时间：
2011-03
影响因子：
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通讯作者：
Pop, Mihai
中科院分区：
文献类型：
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作者：
Treangen, Todd J;Sommer, Dan D;Angly, Florent E;Koren, Sergey;Pop, Mihai
模块化开源汇编器(AMOS)旨在提供一种模块化的基因组组装方法。AMOS包括广泛的组装工具，包括轻量级从头组装程序Minimus和Minimo，以及能够处理元基因组和多态数据的坚固支架BAMbus 2。本协议描述了如何配置和使用AMOS来组装下一代序列数据。此外，我们提供了三个教程示例，其中包括细菌、病毒和元基因组数据集，并提供了提高组装质量的具体提示。
A Modular Open-Source Assembler (AMOS) was designed to offer a modular approach to genome assembly. AMOS includes a wide range of tools for assembly, including lightweight de novo assemblers Minimus and Minimo, and Bambus 2, a robust scaffolder able to handle metagenomic and polymorphic data. This protocol describes how to configure and use AMOS for the assembly of Next Generation sequence data. Additionally, we provide three tutorial examples that include bacterial, viral, and metagenomic datasets with specific tips for improving assembly quality.