The utility of an rTeGM6-4r-based immunochromatographic test for the serological diagnosis of non-tsetse-transmitted equine trypanosomosis in rural areas of Mongolia
The utility of an rTeGM6-4r-based immunochromatographic test for the serological diagnosis of non-tsetse-transmitted equine trypanosomosis in rural areas of Mongolia
复制标题
DOI：
10.1007/s00436-018-5982-8
复制
发表时间：
2018-09-01
影响因子：
2
通讯作者：
Suganuma, Keisuke
中科院分区：
文献类型：
--
作者：
Mizushima, Daiki;Amgalanbaatar, Tovuu;Suganuma, Keisuke
我们之前的研究报告了非采采赛传播的马锥虫病在蒙古的流行。然而，非采采赛传播的马锥虫病在蒙古一些地区的流行现状仍有待澄清。我们先前报道了基于rTeGM6-4r的诊断工具、基于rTeGM6-4r的免疫层析试验(ICT)和酶联免疫吸附试验(ELISA)在蒙古马锥虫病血清学监测中的潜在应用。在本研究中，验证了基于rTeGM6-4r的ICT的实用性。基于rTeGM6-4r的ICT准确诊断了从蒙古牧马中制备的阳性参考血清，类似于基于rTeGM6-4r的酶联免疫吸附试验。当试纸在干燥条件下保存至少2个月时，基于rTeGM6-4R的ICT的诊断性能保持不变。ICT从蒙古所有21个省总共收集的1701份马血清中检测出42份阳性血清样本。与rTeGM6-4r相比，以rTeGM6-4r为基础的ICT的Kappa值、敏感性和特异性分别为0.58、50.0%(95%CI，37.7~62.3%)和99.3%(95%CI，98.7~99.6%)。我们的野外友好型rTeGM6-4R-ICT被发现对蒙古农村地区非采采热传播的马锥虫病的血清学诊断有用。
Our previous studies report epidemics of non-tsetse-transmitted equine trypanosomosis in Mongolia. However, the current status of non-tsetse-transmitted equine trypanosomosis endemicity remains to be clarified in some parts of Mongolia. We previously reported the potential application of rTeGM6-4r-based diagnostic tools, an rTeGM6-4r-based immunochromatographic test (ICT) and an enzyme-linked immunosorbent assay (ELISA), in the serological surveillance of equine trypanosomosis in Mongolia. In the present study, the utility of the rTeGM6-4r-based ICT was validated. The rTeGM6-4r-based ICT accurately diagnosed positive reference sera that had been prepared from dourine horses in Mongolia, similarly to the rTeGM6-4r-based ELISA. The diagnostic performance of the rTeGM6-4r-based ICT was maintained when the strips were preserved for at least 2 months under dry conditions. The ICT detected 42 positive serum samples from a total of 1701 equine sera that had been collected from all 21 provinces of Mongolia. The kappa-value, sensitivity and specificity of rTeGM6-4r-based ICT were 0.58, 50.0% (95% CI, 37.7-62.3%) and 99.3% (95% CI, 98.7-99.6%), respectively, in comparison to the rTeGM6-4r-based ELISA. Our field-friendly rTeGM6-4r-based ICT was found to be useful for the serological diagnosis of non-tsetse-transmitted equine trypanosomosis in rural areas of Mongolia.