Supramolecular nanocarrier of siRNA from PEG-based block catiomer carrying diamine side chain with distinctive pKa directed to enhance intracellular gene silencing
Supramolecular nanocarrier of siRNA from PEG-based block catiomer carrying diamine side chain with distinctive pKa directed to enhance intracellular gene silencing
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DOI：
10.1021/ja047174r
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发表时间：
2004-10-27
影响因子：
15
通讯作者：
Kataoka, K
中科院分区：
文献类型：
--
作者：
Itaka, K;Kanayama, N;Kataoka, K
开发了通过基于PEG的嵌段阳离子聚合物的自组装形成的siRNA纳米载体，所述嵌段阳离子聚合物在侧链中具有两个不同的氨基和不同的pKa值。这种设计为载体提供了足够的siRNA复合物和内体中假定的缓冲能力，使其表现出显著的基因敲低能力以及足够的血清耐受性。
An siRNA nanocarrier formed through self-assembly of PEG-based block catiomer possessing two distinct amino groups with different pKavalues in a side chain was developed. This design provided the carrier with a sufficient siRNA complexation and an assumed buffering capacity in the endosomes, allowing it to exhibit remarkable gene knockdown abilities as well as sufficient serum tolerability.