Cryoembedding and sectioning of cochleas for immunocytochemistry and in situ hybridization
Cryoembedding and sectioning of cochleas for immunocytochemistry and in situ hybridization
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DOI：
10.1016/s1385-299x(00)00048-9
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发表时间：
2001-02-01
期刊：
影响因子：
--
通讯作者：
Greiner, MA
中科院分区：
文献类型：
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作者：
Whitlon, DS;Szakaly, R;Greiner, MA
目前强调生物化学和分子方面的耳蜗解剖强调了高质量的内耳低温切片的必要性。耳蜗内大量的液体空间使得冷冻埋置和器官切片比其他更均匀的组织更困难。我们将耳蜗冷冻包埋用于免疫细胞化学和原位杂交的方法是缓慢浸润，增加蔗糖浓度，然后在最终定向和冷冻前脱气包埋介质。这种方法允许高质量的冷冻切片切割，保持整体结构和细胞分辨率。(C) 2001 Elsevier Science B.V.版权所有
Current emphasis on biochemical and molecular aspects of cochlear anatomy underscores the necessity for high quality cryostat sections of the inner ear. The large volume of fluid space within the cochlea makes cryoembedding and sectioning of the organ more problematic than that of other, more homogeneous tissues. Our method for cryoembedding of cochleas for immunocytochemistry and in situ hybridization uses slow infiltration with increasing concentrations of sucrose followed by degassed embedding medium before final orientation and freezing. This method permits high quality cryosections to be cut which preserve overall structure and cellular resolution. (C) 2001 Elsevier Science B.V. All rights reserved.