Association between colony forming units-granulocyte macrophage expression of Ia-like (HLA-DR) antigen and control of granulocyte and macrophage production. A new role for prostaglandin E.
Association between colony forming units-granulocyte macrophage expression of Ia-like (HLA-DR) antigen and control of granulocyte and macrophage production. A new role for prostaglandin E.
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DOI：
10.1172/jci110649
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发表时间：
1982-09
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影响因子：
--
通讯作者：
L. Pelus
中科院分区：
文献类型：
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作者：
L. Pelus
人粒细胞-巨噬细胞集落形成单位（CFU-GM）上Ia样抗原的表达与细胞周期的S期有关，并与前列腺素E和酸性异铁蛋白在体外对正常粒细胞和巨噬细胞产生的调控有关。CFU-GM的Ia抗原表达在37 ℃培养的3-6小时内丧失，并且与对这些因子的抑制的反应性丧失同时发生。骨髓CFU-GM在有限暴露的悬浮培养物中培养，用1 μ M-1 pM前列腺素E（PGE 1或PGE 2），而不是前列腺素F2 α或二丁酰环-3 '-5'-AMP，24小时后检测到CFU-GM Ia抗原。抗原表达与总CFU-GM和S期CFU-GM的绝对增加以及对前列腺素E和酸性异铁蛋白抑制的反应性恢复相关。在用前列腺素E悬浮培养后，CFU-GM上检测到Ia抗原，这是由于Ia抗原的再表达以及刺激非周期细胞进入S期，表达Ia抗原，并产生对前列腺素E和酸性异铁蛋白抑制敏感的CFU-GM。用前列腺素E悬浮培养后CFU-GM对这些因子抑制的敏感性与悬浮培养前测试的相同细胞的敏感性相同。这些研究为Ia抗原表达和髓系祖细胞分化控制之间的直接调节关联提供了证据，并表明前列腺素E在CFU-GM细胞周期控制、Ia抗原表达和生长调节中的作用。
The expression of Ia-like antigens on human colony forming units-granulocyte macrophage (CFU-GM) is related to S-phase of the cell cycle, and associated with the control of normal granulocyte and macrophage production by prostaglandin E and acidic isoferritins in vitro. Ia-antigen expression by CFU-GM is lost within 3-6 h of culture at 37 degrees C and occurs simultaneously with loss of responsiveness to inhibition by these factors. Culture of bone marrow CFU-GM in a limited exposure suspension culture with 1 microM-1pM prostaglandin E (PGE1 or PGE2), but not prostaglandin F2 alpha or dibutyryl-cyclic-3'-5'-AMP results in the detection of CFU-GM Ia-antigen after 24 h. Antigen expression is associated with an absolute increase in total and S-phase CFU-GM, and restoration of responsiveness to inhibition by prostaglandin E and acidic isoferritins. The detection of Ia-antigen on CFU-GM after suspension culture with prostaglandin E results both from Ia-antigen reexpression as well as stimulation of noncycling cells to enter S-phase, express Ia-antigen and give rise to CFU-GM sensitive to inhibition by prostaglandin E and acidic isoferritins. The sensitivity of CFU-GM to inhibition by these factors after suspension culture with prostaglandin E is identical to that of the same cells tested prior to the suspension culture. These studies provide evidence for a direct regulatory association between Ia-antigen expression and control of myeloid progenitor cell differentiation, and suggest a role for prostaglandin E in the control of CFU-GM cell cycle, Ia-antigen expression, and growth regulation.