Polyelectrolyte multilayer film on decellularized porcine aortic valve can reduce the adhesion of blood cells without affecting the growth of human circulating progenitor cells
Polyelectrolyte multilayer film on decellularized porcine aortic valve can reduce the adhesion of blood cells without affecting the growth of human circulating progenitor cells
复制标题
脱细胞猪主动脉瓣上的聚电解质多层膜可减少血细胞粘附而不影响人循环祖细胞的生长
DOI：
10.1016/j.actbio.2011.11.011
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发表时间：
2012-03-01
影响因子：
9.7
通讯作者：
Zhao, Qiang
中科院分区：
文献类型：
--
作者：
Ye, Xiaofeng;Hu, Xiang;Zhao, Qiang
聚电解质多层膜修饰可有效降低异种支架在体内的免疫和炎症反应，也可应用于组织工程心脏瓣膜与血液接触。本研究的目的是测试肝素-壳聚糖多层膜作为脱细胞心脏主动脉瓣的抗血栓涂层试剂及其修饰后瓣膜表面的生物相容性。扫描电镜观察了血小板的粘附和几何变形。通过测定可溶性p选择素的产生来确定血小板活化的定量分析。此外，白细胞粘附、红细胞溶血和全血凝固时间的研究，以获得该生物材料的血液相容性信息。培养人血源性内皮祖细胞（EPCs），观察EPCs在表面修饰的PDAV上的粘附和生长情况。结果表明，肝素-壳聚糖多层膜可包被在脱细胞瓣膜支架上，改善支架的血液相容性，显著降低血小板粘附和活化。改良后的瓣膜还能显著降低白细胞粘附、红细胞溶血和全血凝固时间。用EPCs播种在脱细胞基质表面形成了一层融合的单层。本工作的体外研究表明，肝素-壳聚糖多层膜作为一种表面修饰手段来改善脱细胞瓣膜支架的血液相容性可能是合理的。(c) 2011材料学报Elsevier Ltd.出版。版权所有。
Polyelectrolyte multilayer film modification could be an effective method to reduce the immunological and inflammatory response of the xenogeneic scaffold in vivo, and may also be applied to tissue-engineered heart valve in contact with blood. The objectives of this study are to test heparin-chitosan multilayer film as an antithrombotic coating reagent for decellularized aortic heart valve and the biocompatibility of the modified valvular surface. The adhesion and geometric deformation of platelets were demonstrated by scanning electron microscopy. The quantitative assay of platelet activation was determined by measuring the production of soluble P-selectin. Moreover, the leukocytes adhesion, erythrocyte hemolysis, and whole blood clotting time studies were performed to gain information on the hemocompatibility of this biomaterial. Human-blood-derived endothelial progenitor cells (EPCs) were cultured and the adhesion and growth of EPCs on the surface-modified PDAV were assessed. The results showed that heparin-chitosan multilayer film could be coated on the decellularized valvular scaffolds, and improved their hemocompatibility with respect to a substantial reduction of platelet adhesion and activation. The modified valve also significantly reduced leukocytes adhesion, erythrocyte hemolysis, and whole blood clotting time. Seeding with EPCs achieved a confluent monolayer on the surface of the decellularized matrix. The in vitro studies performed in this work suggest that it may be reasonable to use heparin-chitosan multilayer film as a means of surface modification to improve the blood compatibility of decellularized valvular scaffold. (c) 2011 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.