Gonadotropin-releasing hormone modulates voltage-activated sodium current and odor responses in Necturus maculosus olfactory sensory neurons.
Gonadotropin-releasing hormone modulates voltage-activated sodium current and odor responses in Necturus maculosus olfactory sensory neurons.
复制标题
促性腺激素释放激素调节 Necturus maculosus 嗅觉感觉神经元的电压激活钠电流和气味反应。
DOI：
10.1002/jnr.21297
复制
发表时间：
2007
影响因子：
4.2
通讯作者：
Delay,RonaJ
中科院分区：
文献类型：
--
作者：
Zhang,Wenling;Delay,RonaJ
末梢神经（末梢神经）从基底前脑延伸到鼻腔，已被证明含有促性腺激素释放激素（GnRH）。末梢神经的具体功能尚不清楚，但据推测，它调节嗅觉神经元的功能。为了研究GnRH对分离的斑状内痣嗅觉感觉神经元（OSNs）的影响，我们使用膜片钳技术的穿孔配置来记录电流反应。GnRH对任何保持电位下的膜电流都没有影响，但可以调节电压激活的TTX敏感钠电流（INa）。在应用GnRH后1分钟内，约60%的osn显示出INa幅度的下降。对GnRH的初始反应是抑制性的，尽管在一组细胞中，初始抑制反应之后持续应用ina（~ 5分钟）。GnRH反应的时间过程表明，第二信使途径介导了反应。PKC、酪氨酸激酶和PI3K抑制剂均能抑制INa，但均不能阻止GnRH反应。cAMP类似物的应用模拟了GnRH的作用，只有PKA和PKG抑制剂可以阻止GnRH诱导的INa抑制。这表明GnRH对电压激活钠电流的调节涉及环核苷酸途径。此外，GnRH调节了osn的气味反应。我们的数据表明GnRH的释放，可能来自末梢神经，可以调节嗅觉感觉神经元。©2007 Wiley‐Liss, Inc。
The terminal nerve (nervus terminalis) extends from the basal forebrain to the nasal cavity and has been shown to contain gonadotropin‐releasing hormone (GnRH). The specific function of the terminal nerve is unknown, but it has been hypothesized that it modulates the function of olfactory neurons. To examine the effects of GnRH on isolatedNecturus maculosusolfactory sensory neurons (OSNs), we used the perforated configuration of the patch clamp technique to record current responses. GnRH had no effect on the membrane current at any holding potential but did modulate voltage‐activated TTX‐sensitive sodium current (INa). Within 1 min of applying GnRH, approximately 60% of the OSNs showed a decrease in the magnitude of INa. Initial responses to GnRH were inhibitory, although in one group of cells the initial inhibitory response was followed by a potentiation of INawith continual application (∼5 min). The time course of the GnRH response suggested that a second messenger pathway mediated the response. Inhibitors of PKC, tyrosine kinase, and PI3K were all able to inhibit the INa, but none of them could prevent the GnRH response. Application of a cAMP analog mimicked the effects of GnRH, and only inhibitors of PKA and PKG could prevent GnRH‐induced inhibition of INa. This suggests that the modulation of voltage‐activated sodium currents by GnRH involve a cyclic nucleotide pathway. In addition, GnRH modulated the odor responses of OSNs. Our data suggest the release of GnRH, presumably from the terminal nerve, can serve to modulate olfactory sensory neurons. © 2007 Wiley‐Liss, Inc.