Exploring Cell Wall Composition and Modifications During the Development of the Gynoecium Medial Domain in Arabidopsis
Exploring Cell Wall Composition and Modifications During the Development of the Gynoecium Medial Domain in Arabidopsis
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DOI：
10.3389/fpls.2018.00454
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发表时间：
2018-04-12
影响因子：
5.6
通讯作者：
de Folter, Stefan
中科院分区：
文献类型：
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作者：
Herrera-Ubaldo, Humberto;de Folter, Stefan
在拟南芥中，雌蕊，花的内轮，是雌性生殖部分。许多对受精很重要的组织，如柱头、花柱、传送道、胎座、胚珠和间隔，包括内侧域，都是由心皮边缘分生组织产生的。在雌蕊发育过程中，隔融合发生，组织不断形成，为成功授粉和受精做准备。在雌蕊的发育过程中，细胞壁发生变化，其中最重要的是传递道的形成，它促进了花粉管的生长和通过卵巢的运动，对生殖能力有很大的影响。在这项研究中，我们使用经典染色方法、荧光染料和间接免疫定位相结合的方法，分析了雌蕊发育过程中细胞壁的组成和伴随内侧结构域形成的修饰。我们发现多糖分布随时间的协调变化，传递道形成前细胞壁的修饰，传递道形成过程中黏液物质增加，甘露聚糖分布减少。此外，我们还检测到了中隔融合过程中脂质分布的变化。适当的细胞壁组成和修饰对于心皮的生殖后融合（隔膜融合）和传递道的形成是重要的，因为这些组织影响植物的生殖能力。
In Arabidopsis, the gynoecium, the inner whorl of the flower, is the female reproductive part. Many tissues important for fertilization such as the stigma, style, transmitting tract, placenta, ovules, and septum, comprising the medial domain, arise from the carpel margin meristem. During gynoecium development, septum fusion occurs and tissues form continuously to prepare for a successful pollination and fertilization. During gynoecium development, cell wall modifications take place and one of the most important is the formation of the transmitting tract, having a great impact on reproductive competence because it facilitates pollen tube growth and movement through the ovary. In this study, using a combination of classical staining methods, fluorescent dyes, and indirect immunolocalization, we analyzed cell wall composition and modifications accompanying medial domain formation during gynoecium development. We detected coordinated changes in polysaccharide distribution through time, cell wall modifications preceding the formation of the transmitting tract, mucosubstances increase during transmitting tract formation, and a decrease of mannan distribution. Furthermore, we also detected changes in lipid distribution during septum fusion. Proper cell wall composition and modifications are important for postgenital fusion of the carpel (septum fusion) and transmitting tract formation, because these tissues affect plant reproductive competence.