Altered profiles of intestinal microbiota and organic acids may be the origin of symptoms in irritable bowel syndrome
Altered profiles of intestinal microbiota and organic acids may be the origin of symptoms in irritable bowel syndrome
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DOI：
10.1111/j.1365-2982.2009.01427.x
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发表时间：
2010-05-01
影响因子：
3.5
通讯作者：
Fukudo, S.
中科院分区：
文献类型：
--
作者：
Tana, C.;Umesaki, Y.;Fukudo, S.
背景肠易激综合征（IBS）患者肠道有机酸的变化及其与胃肠道症状的关系尚不清楚。我们假设在这项研究中，改变胃肠道菌群有助于IBS症状通过增加水平的有机acids.MethodsSubjects 26 IBS患者和26年龄和性别匹配的对照。收集粪便样本，使用定量实时聚合酶链反应和培养方法进行微生物群分析，并使用高效液相色谱法测定有机酸水平。腹部气体通过腹部X线片的图像分析来量化。受试者完成了一份问卷调查的胃肠道症状，生活质量（QOL）和消极emotive.Key Results.Key结果肠易激综合征患者的韦荣氏球菌（P = 0.046）和乳酸杆菌（P = 0.031）比对照组显着较高的计数。与对照组相比，它们还表达了显著更高水平的乙酸（P = 0.049）、丙酸（P = 0.025）和总有机酸（P = 0.014）。肠气体量在对照组和IBS患者之间无显著差异。最后，高乙酸或丙酸水平的IBS患者的胃肠道症状，生活质量和负面情绪比那些低乙酸或丙酸水平或controls.Conclusions & InferencesThese结果支持这一假设，即粪便微生物群和有机酸改变IBS患者。已知韦荣氏球菌和乳杆菌的组合产生乙酸和丙酸。高水平的乙酸和丙酸可能与IBS患者的腹部症状、生活质量受损和负面情绪有关。
BackgroundThe profile of intestinal organic acids in irritable bowel syndrome (IBS) and its correlation with gastrointestinal (GI) symptoms are not clear. We hypothesized in this study that altered GI microbiota contribute to IBS symptoms through increased levels of organic acids.MethodsSubjects were 26 IBS patients and 26 age- and sex-matched controls. Fecal samples were collected for microbiota analysis using quantitative real-time polymerase chain reaction and culture methods, and the determination of organic acid levels using high-performance liquid chromatography. Abdominal gas was quantified by image analyses of abdominal X-ray films. Subjects completed a questionnaire for GI symptoms, quality of life (QOL) and negative emotion.Key ResultsIrritable bowel syndrome patients showed significantly higher counts of Veillonella (P = 0.046) and Lactobacillus (P = 0.031) than controls. They also expressed significantly higher levels of acetic acid (P = 0.049), propionic acid (P = 0.025) and total organic acids (P = 0.014) than controls. The quantity of bowel gas was not significantly different between controls and IBS patients. Finally, IBS patients with high acetic acid or propionic acid levels presented with significantly worse GI symptoms, QOL and negative emotions than those with low acetic acid or propionic acid levels or controls.Conclusions & InferencesThese results support the hypothesis that both fecal microbiota and organic acids are altered in IBS patients. A combination of Veillonella and Lactobacillus is known to produce acetic and propionic acid. High levels of acetic and propionic acid may associate with abdominal symptoms, impaired QOL and negative emotions in IBS.