Absorption and metabolism of 4-hydroxyderricin and xanthoangelol after oral administration of Angelica keiskei (Ashitaba) extract in mice.
Absorption and metabolism of 4-hydroxyderricin and xanthoangelol after oral administration of Angelica keiskei (Ashitaba) extract in mice.
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DOI：
10.1016/j.abb.2012.03.013
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发表时间：
2012-05
影响因子：
3.9
通讯作者：
Toshiyuki Nakamura;Takahiro Tokushima;K. Kawabata;Norio Yamamoto;M. Miyamoto;H. Ashida
中科院分区：
文献类型：
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作者：
Toshiyuki Nakamura;Takahiro Tokushima;K. Kawabata;Norio Yamamoto;M. Miyamoto;H. Ashida
为了研究4-羟基槲皮素和黄樟醇的吸收和代谢，我们建立了一种基于液相色谱-串联质谱的分析方法，并测定了这些化合物在小鼠口服Ashitaba提取物（50- 500 mg/kg体重）后的血浆、尿液、粪便、肝脏、肾脏、脾脏、肌肉和白色脂肪组织中的含量。4-羟基德里克星和黄当归醇很快被吸收到血浆中，4-羟基德里克星和黄当归醇达到最大血浆浓度的时间分别为2小时和0.5小时。虽然这些化合物具有相似的结构，但在24小时时，4-羟基derricin及其代谢物的总血浆浓度约为xanthoangelol及其代谢物的4倍。4-羟基德里克星和黄当归醇主要以其糖苷配基形式和相关代谢产物（葡萄糖醛酸和/或硫酸盐形式）在口服Ashitaba提取物后2 - 4 h内经尿液排泄。另一方面，这些化合物仅以其糖苷配基形式在粪便中排泄。当在施用Ashitaba提取物后2小时估计4-羟基derricin和黄樟醇的组织分布时，在评估的所有组织中检测到这两种化合物，主要以其糖苷配基形式，除了在肠系膜脂肪组织中。这些结果表明，4-羟基derricin和xanthoangelol被迅速吸收和分布到各种组织。
To investigate the absorption and metabolism of 4-hydroxyderricin and xanthoangelol, we established an analytical method based on liquid chromatography-tandem mass spectrometry and measured these compounds in the plasma, urine, feces, liver, kidney, spleen, muscle and white adipose tissues of mice orally administered with Ashitaba extract (50–500mg/kg body weight). 4-Hydroxyderricin and xanthoangelol were quickly absorbed into the plasma, with time-to-maximum plasma concentrations of 2 and 0.5h for 4-hydroxyderricin and xanthoangelol, respectively. Although these compounds have similar structures, the total plasma concentration of 4-hydroxyderricin and its metabolites was approximately 4-fold greater than that of xanthoangelol and its metabolites at 24h. 4-Hydroxyderricin and xanthoangelol were mostly excreted in their aglycone forms and related metabolites (glucuronate and/or sulfate forms) in urine between 2 and 4h after oral administration of Ashitaba extract. On the other hand, these compounds were only excreted in their aglycone forms in feces. When tissue distribution of 4-hydroxyderricin and xanthoangelol was estimated 2h after administration of Ashitaba extract, both compounds were detected in all of the tissues assessed, mainly in their aglycone forms, except in the mesenteric adipose tissue. These results suggest that 4-hydroxyderricin and xanthoangelol are rapidly absorbed and distributed to various tissues.