A synthetic icosahedral DNA-based host-cargo complex for functional in vivo imaging
A synthetic icosahedral DNA-based host-cargo complex for functional in vivo imaging
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DOI：
10.1038/ncomms1337
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发表时间：
2011-06-01
影响因子：
16.6
通讯作者：
Krishnan, Yamuna
中科院分区：
文献类型：
--
作者：
Bhatia, Dhiraj;Surana, Sunaina;Krishnan, Yamuna
将分子货物包封在明确定义的超分子结构内是非常具有挑战性的。合成宿主是理想的，因为它们具有明确定义的性质和可寻址性。在合成宿主内包封生物大分子尤其具有挑战性，因为前者的大尺寸、敏感性、包封后功能的保留以及对货物的控制的证明。在这里，我们封装的荧光生物聚合物，作为一个pH值报告内的合成，基于DNA的二十面体主机主机没有主机和货物之间的分子识别。只有那些携带宿主-货物复合物的DNA外壳受体的细胞才能吞噬它。因此，我们证明了包囊货物是秀丽隐杆线虫细胞特异性摄取的。通过空间映射与C.优美的这是第一次证明合成的宿主-货物复合物在体内的功能和紧急行为。
The encapsulation of molecular cargo within well-defined supramolecular architectures is highly challenging. Synthetic hosts are desirable because of their well-defined nature and addressability. Encapsulation of biomacromolecules within synthetic hosts is especially challenging because of the former's large size, sensitive nature, retention of functionality post-encapsulation and demonstration of control over the cargo. Here we encapsulate a fluorescent biopolymer that functions as a pH reporter within synthetic, DNA-based icosahedral host without molecular recognition between host and cargo. Only those cells bearing receptors for the DNA casing of the host-cargo complex engulf it. We show that the encapsulated cargo is therefore uptaken cell specifically in Caenorhabditis elegans. Retention of functionality of the encapsulated cargo is quantitatively demonstrated by spatially mapping pH changes associated with endosomal maturation within the coelomocytes of C. elegans. This is the first demonstration of functionality and emergent behaviour of a synthetic host-cargo complex in vivo.