Friend virus-specific cytotoxic T lymphocytes recognize both gag and env gene-encoded specificities.
Friend virus-specific cytotoxic T lymphocytes recognize both gag and env gene-encoded specificities.
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Friend 病毒特异性细胞毒性 T 淋巴细胞可识别 gag 和 env 基因编码的特异性。
DOI：
10.1084/jem.164.1.211
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Lilly,F
中科院分区：
文献类型：
--
作者：
Holt,CA;Osorio,K;Lilly,F
材料和方法所有小鼠均在Albert Einstein College of Medicine，布朗克斯，NY饲养。所用近交系包括H-2同源系BALB/cAn（H-2d）、BALB/cAn（H-2d）和BALB/cAn（H-2d）。B（H-2 B）、BALB。G（H-2g）和BALB. 5R（H-2'5）。在这些研究中使用6-12周龄的雌性小鼠。Fisher大鼠胚胎（FRE）克隆2成纤维细胞（14）用作构建靶细胞的亲本细胞系。SFFVv-FRE是通过终点稀释用源自红细胞增多症诱导性FV的Lilly-Steeves株的SFFV的生物克隆非生产性感染的FRE细胞（15），EY-10是用SFFVv和源自贫血诱导性FV株的FMuLVA的生物克隆两者生产性感染的FRE细胞（16）。平行维持仅用FMuLVA生产性感染的细胞系（Fz 0 ~ NIH）（17）作为克隆辅助病毒的来源，其可用于感染FRE细胞。化学诱导的C57 BL/6淋巴瘤细胞系EL 4用作14-2 B对照。所有品系在补充有10%FCS、谷氨酰胺和抗生素的DME中生长。
Materials and Methods Mice. All mice were bred at the Albert Einstein College of Medicine, Bronx, NY. The inbred strains used included the H-2-congenic strains BALB/cAn (H-2d), BALB. B (H-2b), BALB. G (H-2g), and BALB. 5R (H-2'5). Female mice aged 6-12 wk were used in these studies.Cells and Viruses. Fisher rat embryo (FRE) clone 2 fibroblasts (14) were used as the parental cell line for the construction of target cells. SFFVv-FRE are FRE cells nonproductively infected with a biological clone of SFFV derived from the Lilly-Steeves strain of polycythemia-inducing FV (15) by end-point dilution, and EY-10 are FRE cells productively infected with both SFFVv and a biological clone of FMuLVA derived from an anemia-inducing strain of FV (16). A cell line productively infected with FMuLVA only (Fz0~ NIH)(17) was maintained in parallel as a source of cloned helper virus, which could be used to infect FRE cells. The chemically induced C57BL/6 lymphoma cell line EL4 served as an 14-2 b control. All lines were grown in DME supplemented with 10% FCS, glutamine, and antibiotics.