Exacerbation of chronic aminonucleoside nephrosis by dietary cholesterol supplementation.
Exacerbation of chronic aminonucleoside nephrosis by dietary cholesterol supplementation.
复制标题
膳食胆固醇补充剂加剧慢性氨基核苷肾病。
DOI：
10.1038/ki.1987.259
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发表时间：
1987
影响因子：
19.6
通讯作者：
Karnovsky,MJ
中科院分区：
文献类型：
--
作者：
Diamond,JR;Karnovsky,MJ
膳食胆固醇补充剂加剧慢性氨基核苷肾病。肾病综合征引起的脂质代谢异常可能在最初的肾小球损伤发展为局灶性和节段性肾小球硬化（FSGS）的过程中发挥作用。为了更具体地评估这一点，我们喂养雄性 Sprague-Dawley 大鼠，通过单次静脉注射嘌呤霉素氨基核苷 (PA) 使肾病，无论是正常啮齿动物饲料（第 1 组）还是添加 4% 胆固醇/1% 胆酸的相同制剂（第 2 组）。使用这种添加 4% 胆固醇/1% 胆酸的饮食是因为，在正常的非肾病大鼠中，这种营养补充剂在很大程度上只会导致空腹血清胆固醇显着升高，而不会显着升高空腹血清甘油三酯。 PA 分娩后 18 周出现 FSGS，并对两组大鼠进行功能和形态学研究。与第1组动物相比，第2组大鼠的每日尿蛋白排泄量显着更高，菊粉清除率更低，血液尿素氮浓度更高。从组织学角度来看，第 2 组动物表现出显着更高的肾小球百分比，其肾小球硬化/透明变性、系膜细胞增殖和系膜“泡沫”的节段区域被检查；细胞。在 PA 给药后 2、4、12 和 18 周，第 2 组大鼠的空腹血清胆固醇始终显着升高，而就空腹血清甘油三酯而言，第 2 组大鼠仅在 PA 给药后第 4 周和 12 周显着升高。
Exacerbation of chronic aminonucleoside nephrosis by dietary cholesterol supplementation. Abnormalities in lipid metabolism resulting from nephrotic syndrome may play a role in the progression of initial glomerular injury to focal and segmental glomerulosclerosis (FSGS). In order to more specifically assess this, we fed male Sprague–Dawley rats, made nephrotic with a single intravenous injection of puromycin aminonucleoside (PA), either normal rodent chow (Group 1) or the same formulation supplemented with 4% cholesterol/1% cholic acid (Group 2). This 4% cholesterol/1% cholic acid–added diet was utilized because, in normal, non-nephrotic rats, this alimentary supplement produces, for the most part, only a significant rise in fasting serum cholesterol and not fasting serum triglycerides. FSGS developed 18 weeks after PA delivery, and both groups of rats were studied functionally and morphologically. Group 2 rats had significantly higher daily urine protein excretion, lower inulin clearance, and greater blood urea nitrogen concentrations than Group 1 animals. Histologically, Group 2 animals demonstrated a significantly greater percentage of glomeruli examined with segmental areas of glomerulosclerosis/hyalinosis, mesangial cell proliferation, and mesangial “foam”; cells. At 2, 4, 12, and 18 weeks after PA delivery, the fasting serum cholesterol was always significantly greater in Group 2 rats, whereas in regards to fasting serum triglycerides it was only significantly elevated in Group 2 rats at 4 and 12 weeks after PA administration.