An Efficient Glycoblotting-Based Analysis of Oxidized Lipids in Liposomes and a Lipoprotein
An Efficient Glycoblotting-Based Analysis of Oxidized Lipids in Liposomes and a Lipoprotein
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DOI：
10.1002/cbic.201700384
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发表时间：
2017-10-05
期刊：
影响因子：
3.2
通讯作者：
Hui, Shu-Ping
中科院分区：
文献类型：
--
作者：
Furukawa, Takayuki;Hinou, Hiroshi;Hui, Shu-Ping
虽然广泛发生的脂质氧化，这是由活性氧（ROS）触发，产生各种氧化脂质，有效地分析氧化脂质的实用方法仍然难以捉摸。在本文中，表明糖印迹平台可用于分析氧化脂质。分析是基于这样的原理，即脂质醛，氧化脂质物质之一，可以被选择性地捕获，富集和检测。此外，3-甲基-1-对甲苯基三氮烯（MTT）甲基化的磷酸和羧酸，这种MTT介导的甲基化，与传统的串联质谱（MS/MS）分析相结合，氧化脂质的结构分析的有效方法。通过使用三类标准品，脂质体和脂蛋白，证明了糖印迹法是一种强大的方法，即使在复杂的大分子中，也可以用于聚焦脂质组学。
Although widely occurring lipid oxidation, which is triggered by reactive oxygen species (ROS), produces a variety of oxidized lipids, practical methods to efficiently analyze oxidized lipids remain elusive. Herein, it is shown that the glycoblotting platform can be used to analyze oxidized lipids. Analysis is based on the principle that lipid aldehydes, one of the oxidized lipid species, can be captured selectively, enriched, and detected. Moreover, 3-methyl-1-p-tolyltriazene (MTT) methylates phosphoric and carboxylic acids, and this MTT-mediated methylation is, in combination with conventional tandem mass spectrometry (MS/MS) analysis, an effective method for the structural analysis of oxidized lipids. By using three classes of standards, liposomes, and a lipoprotein, it is demonstrated that glycoblotting represents a powerful approach for focused lipidomics, even in complex macromolecules.