TANK, a co-inducer with TRAF2 of TNF- and CD40L-mediated NF-kappa B activation
TANK, a co-inducer with TRAF2 of TNF- and CD40L-mediated NF-kappa B activation
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DOI：
10.1101/gad.10.8.963
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发表时间：
1996-04-15
影响因子：
10.5
通讯作者：
Baltimore, D
中科院分区：
文献类型：
--
作者：
Cheng, GH;Baltimore, D
我们描述了一种新的NF-κ B B激活的信号介质，TANK，它在两种表面受体CD 40和TNER II共同的通路中起作用。TRAF家族成员直接与这些受体相互作用。使用酵母双杂交系统，TANK被鉴定为与所有三个已知TRAF家族成员相互作用的细胞内蛋白，没有以前的同源物。在共转染实验中，TANK和TRAE 2协同激活NF-κ B，需要TANK的氨基末端部分和TRAF 2的环指结构域。TANK具有负作用的羧基末端，并且是组成性无活性的，但是TRAE 2结合克服了内部抑制影响。我们认为配体与CD 40或TNFR II结合导致TRAE 2/TANK复合物的形成，介导NP-κ B活化。
We describe a new signal mediator of NF-kappa B activation, TANK, that acts in a pathway common to two surface receptors CD40 and TNER II. TRAF family members interact directly with these receptors. Using the yeast two-hybrid system, TANK was identified as an intracellular protein without previous homologs that interacts with all three known TRAF family members. In cotransfection experiments, TANK and TRAE2 activate NF-kappa B synergistically, requiring both the amino-terminal portion of TANK and the ring finger domain of TRAF2. TANK has a negatively acting carboxyl terminus and is constitutively inactive, but TRAE2 binding overcomes the internal inhibitory influence. We propose that ligand binding to CD40 or TNFR II leads to the formation of a TRAE2/TANK complex, mediating NP-kappa B activation.