Use of DNA barcodes to identify flowering plants
Use of DNA barcodes to identify flowering plants
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DOI：
10.1073/pnas.0503123102
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发表时间：
2005-06-07
影响因子：
11.1
通讯作者：
Janzen, DH
中科院分区：
文献类型：
--
作者：
Kress, WJ;Wurdack, KJ;Janzen, DH
通过使用被称为“DNA条形码”的短直系同源DNA序列来识别物种的方法已经被提出并启动，以促进生物多样性研究、识别幼体、关联性别以及加强法医分析。细胞色素c氧化酶1序列已被发现广泛适用于动物条形码，但由于高等植物中细胞色素c氧化酶1基因的进化速度比动物慢得多，它并不适用于大多数植物物种。因此，我们提出核内转录间隔区和质体trnH - psbA基因间间隔区作为可用于开花植物条形码的潜在DNA区域。内转录间隔区是在植物物种水平的系统发育研究中最常测序的基因座，并且显示出高水平的种间差异。trnH - psbA间隔区虽然很短（约450bp），但却是被子植物中变异最大的质体区域，并且很容易在广泛的陆生植物中扩增。对烟草和颠茄的总质体基因组进行比较，并在差异很大的被子植物类群上进行试验（包括7个植物科的近缘物种以及从涵盖50个植物科的当地植物区系中采集的一组物种，总共99个物种、80个属和53个科），结果表明这对基因座中的序列有可能为条形码目的区分最多数量的植物物种。
Methods for identifying species by using short orthologous DNA sequences, known as "DNA barcodes," have been proposed and initiated to facilitate biodiversity studies, identify juveniles, associate sexes, and enhance forensic analyses. The cytochrome c oxidase 1 sequence, which has been found to be widely applicable in animal barcoding, is not appropriate for most species of plants because of a much slower rate of cytochrome c oxidase 1 gene evolution in higher plants than in animals. We therefore propose the nuclear internal transcribed spacer region and the plastid trnH-psbA intergenic spacer as potentially usable DNA regions for applying barcoding to flowering plants. The internal transcribed spacer is the most commonly sequenced locus used in plant phylogenetic investigations at the species level and shows high levels of interspecific divergence. The trnH-psbA spacer, although short (approximate to 450-bp), is the most variable plastid region in angiosperms and is easily amplified across a broad range of land plants. Comparison of the total plastid genomes of tobacco and deadly nightshade enhanced with trials on widely divergent angiosperm taxa, including closely related species in seven plant families and a group of species sampled from a local flora encompassing 50 plant families (for a total of 99 species, 80 genera, and 53 families), suggest that the sequences in this pair of loci have the potential to discriminate among the largest number of plant species for barcoding purposes.