N-acetyl cysteine pre-treatment attenuates inflammatory changes in the warm ischemic murine lung
N-acetyl cysteine pre-treatment attenuates inflammatory changes in the warm ischemic murine lung
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DOI：
10.1016/j.healun.2007.09.008
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发表时间：
2007-12-01
影响因子：
8.9
通讯作者：
Van Raemdonck, Dirk E. M.
中科院分区：
文献类型：
--
作者：
Geudens, Nele;De Wauwer, Caroline Van;Van Raemdonck, Dirk E. M.
背景资料：无心跳供体肺的热缺血期可能是肺移植术后更严重的缺血再灌注损伤的原因之一。我们研究了N-乙酰半胱氨酸（NAC）给药对炎症参数的影响和时机。方法：通过夹闭左肺门90分钟诱导缺血（1），然后再灌注（R）4小时。将小鼠分为9组（n = 6/组）：3个对照组（[假手术]）（胸廓切开术。仅）、[1]和[I+R]）;两组仅滴注生理盐水（[生理盐水+I]和[生理盐水+I+R]）;四个实验组通过滴注（[NAC + I]、[NAC + I + R]和[I + NAC + R]）或气雾剂（[NACaero + I + R]）给予NAC（50 mg/kg）。结果：肺门阻断前给予NAC可显著降低巨噬细胞、淋巴细胞和白细胞介素1 β（IL-1 β）水平。NAC也导致显着减少巨噬细胞，淋巴细胞和中性粒细胞，以及IL-1 β，角质形成细胞因子（KC），单核细胞趋化蛋白（MCP）-1和IL-6水平在BAL后reperfusion.Conclusions：NAC治疗前热缺血减弱缺血和再灌注期后的炎症变化。心肺移植杂志2007;26：1326-32.版权所有（c）2007国际心肺移植学会。
Background: The warm ischemic period in non-heart-beating donor lungs may contribute to a higher degree of ischemia-reperfusion injury after lung transplantation. We investigated the impact and timing of administration of N-acetyl cysteine (NAC) on inflammatory parameters.Methods: Ischemia (1) was induced by clamping the hilum of the left lung for 90 minutes, and some protocols were followed by reperfusion (R) for 4 hours. Mice were divided into nine groups (n = 6/group): three control groups ([sham] (thoracotomy. only), [1] and [I+R]); two groups with saline instillation only ([saline+I] and [saline+I+R]); and four experimental groups with NAC (50 mg/kg), administered by instillation ([NAC + I], [NAC + I + R] and [I + NAC + R]) or by aerosol ([NACaero + I + R]). Cell counts and protein levels in bronchoalveolar lavage (BAL) were determined.Results: NAC administered prior to hilar clamping led to a significant decrease in macrophages and lymphocytes and interleukin (IL-1 beta levels after ischemia. NAC also resulted in significantly fewer macrophages, lymphocytes and neutrophils as well as IL-1 beta, keratinocyte cytokine (KC), monocyte chemoattractant protein (MCP)-1 and IL-6 levels in BAL taken after reperfusion.Conclusions: NAC treatment prior to warm ischemia attenuates inflammatory changes after both the ischemic and reperfusion periods. J Heart Lung Transplant 2007;26:1326-32. Copyright (c) 2007 by the International Society for Heart and Lung Transplantation.