Interleukin-15 inhibits smooth muscle cell proliferation and hyaluronan production in rat ductus arteriosus
Interleukin-15 inhibits smooth muscle cell proliferation and hyaluronan production in rat ductus arteriosus
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DOI：
10.1203/pdr.0b013e31813c9339
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发表时间：
2007-10-01
影响因子：
3.6
通讯作者：
Yokota, Shumpei
中科院分区：
文献类型：
--
作者：
Iwasaki, Shiho;Minamisawa, Susumu;Yokota, Shumpei
新生内膜垫形成（NCF）是动脉导管（DA）解剖闭合过程中的重要血管重塑。已知对血管损伤或动脉粥样硬化的炎症反应与NCF的发病机制相关。我们发现，白细胞介素（IL）-15 mRNA的表达显着高于在大鼠DA比在主动脉。IL-15主要表达于大鼠DA的内弹力层（IEL），其次为平滑肌细胞（SMCs）。前列腺素E（PGE）可增加培养的DA SMCs中IL-15 mRNA的表达。IL-15显著减弱血小板衍生生长因子（PDGF）-BB介导的SMC增殖，但不改变SMC迁移。IL-15以剂量依赖性方式显著减弱PGE（1）诱导的透明质酸（HA）产生，透明质酸是NCF的有效刺激剂。因此，IL-15可能对关闭DA的生理性血管重塑过程具有抑制作用。
Neointimal cushion formation (NCF) is an important vascular remodeling for anatomical closure of the ductus arteriosus (DA). Inflammatory responses to vascular injury or atherosclerosis are known to be associated with the pathogenesis of NCF. We found that the expression of interleukin (IL)-15 mRNA was significantly higher in rat DA than in the aorta. IL-15 immunoreactivity was detected predominantly in the internal elastic laminae (IEL) and to a lesser extent in smooth muscle cells (SMCs) in rat DA. Prostaglandin E (PGE) increased the expression of IL-15 mRNA in cultured DA SMCs. IL-15 significantly attenuated the platelet-derived growth factor (PDGF)-BB-mediated SMC proliferation, but did not change SMC migration. IL-15 significantly attenuated PGE(1)-induced hyaluronic acid (HA) production in a dose-dependent manner, which is a potent stimulator of NCF. Accordingly, IL-15 might have an inhibitory effect on the physiologic vascular remodeling processes in closing the DA.