&ITPorphyromonas gingivalis&IT, a periodontitis causing bacterium, induces memory impairment and age-dependent neuroinflammation in mice
&ITPorphyromonas gingivalis&IT, a periodontitis causing bacterium, induces memory impairment and age-dependent neuroinflammation in mice
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DOI：
10.1186/s12979-017-0110-7
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发表时间：
2018-01-30
影响因子：
7.9
通讯作者：
Zhou, Yanmin
中科院分区：
文献类型：
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作者：
Ding, Ye;Ren, Jingyi;Zhou, Yanmin
背景：据报道，牙周炎和阿尔茨海默病（AD）之间可能存在联系。然而，关于牙龈卟啉单胞菌（P.gingivalis）牙周感染与 AD 病理特征之间关系的信息有限。测试了牙龈卟啉单胞菌牙周感染可能通过年龄依赖性神经炎症导致认知障碍的假设。结果：30只4周龄（幼龄）雌性C57BL/6 J小鼠随机分为两组，对照组和实验组。 30 名 12 个月大的婴儿（中年）如上所述分组。实验组小鼠的口腔感染了牙龈卟啉单胞菌。 6周后进行Morris水迷宫（MWM）评估小鼠的学习记忆能力。此外，通过实时定量聚合酶链反应（qRT-PCR）、酶联免疫吸附测定（ELISA）和免疫组织化学法测定小鼠脑组织中促炎细胞因子TNF-α、IL-6和IL-1β的表达水平。我们的结果表明，感染牙龈卟啉单胞菌的中年小鼠的学习和记忆能力受到损害。此外，中年牙龈卟啉单胞菌感染小鼠脑组织中促炎细胞因子TNF-α、IL-6和IL-1β的表达水平升高。 结论：牙龈卟啉单胞菌牙周感染可能通过中年小鼠脑组织中促炎细胞因子TNF-α、IL-6和IL-1βR的释放而导致认知障碍。
Background: A possible relationship between periodontitis and Alzheimer's disease (AD) has been reported. However, there is limited information on the association between the Porphyromonas gingivalis (P. gingivalis) periodontal infection and the pathological features of AD. The hypothesis that P. gingivalis periodontal infection may cause cognitive impairment via age-dependent neuroinflammation was tested.& para;& para;Results: Thirty 4-week-old (young) female C57BL/6 J mice were randomly divided into two groups, the control group and the experimental group. Thirty 12-month-old (middle-aged) were grouped as above. The mouth of the mice in the experimental group was infected with P. gingivalis. Morris water maze(MWM) was performed to assess the learning and memory ability of mice after 6 weeks. Moreover, the expression levels of the pro-inflammatory cytokines TNF-alpha, IL-6, and IL-1 beta in the mice brain tissues were determined by Quantitative real-time polymerase chain reaction (qRT-PCR), Enzyme Linked Immunosorbent Assay(ELISA) and immunohistochemistry. Our results showed that the learning and memory abilities of the middle-aged P. gingivalis infected mice were impaired. Moreover, the expression levels of the pro-inflammatory cytokines TNF-alpha, IL-6, and IL-1 beta in the brain tissues of the middle-aged P. gingivalis infected mice were increased.& para;& para;Conclusions: These results suggest that P. gingivalis periodontal infection may cause cognitive impairment via the release of the pro-inflammatory cytokines TNF-alpha, IL-6, and IL-1 beta R in the brain tissues of middle-aged mice.