Prevalence of MCPyV in Merkel cell carcinoma and non-MCC tumors
Prevalence of MCPyV in Merkel cell carcinoma and non-MCC tumors
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DOI：
10.1111/j.1600-0560.2009.01352.x
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发表时间：
2010-01-01
影响因子：
1.7
通讯作者：
Flaig, Michael J.
中科院分区：
文献类型：
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作者：
Andres, Christian;Belloni, Benedetta;Flaig, Michael J.
背景默克尔细胞多瘤病毒（MCPyV）可能是默克尔细胞癌（MCC）的病原体。然而，MCPyV 在非 MCC 人群中的患病率及其在其他皮肤癌发病机制中的可能作用尚不清楚。方法对 33 个 MCC 样本和 33 个年龄和性别匹配的阳光照射非 MCC 肿瘤样本进行分子病理学研究 [12 例脂溢性角化病 (SK)、11 例基底细胞癌 (BCC) 和 10 例恶性雀斑样痣] 黑色素瘤（LMM）]。通过聚合酶链式反应 (PCR) 和 PCR 产物 Southern Blot 杂交分析所有肿瘤中 MCPyV-DNA 的存在。结果在 21 个 MCC 样本 (64%) 和 2 个阳光照射皮肤的非 MCC 肿瘤 (6%；均为 SK 患者) 中检测到 MCPyV 序列。 BCC 和 LMM 的组织样本均未证明 MCPyV 序列呈阳性。结论我们能够确认 MCC 中 MCPyV-DNA 流行率的先前数据。此外，检测到 MCPyV 阳性 MCC 患者以女性为主。 MCPyV 与 SK、BCC 和 LMM 没有相关关联。据推测，年龄和性别匹配的非 MCC 人群中 MCPyV 的患病率平均可达 6%。Andres C、Belloni B、Puchta U、Sander CA、Flaig MJ。默克尔细胞多瘤病毒是否也普遍存在于阳光照射皮肤的非默克尔细胞癌 (MCC) 肿瘤中？一项针对 66 名患者的研究。
BackgroundMerkel cell polyomavirus (MCPyV) is the likely causative agent of Merkel cell carcinoma (MCC). However, the prevalence of MCPyV in non-MCC population and its possible role in the pathogenesis of other skin cancers are not known yet.MethodsA molecular pathology study was performed in 33 MCC samples and 33 age- and sex-matched samples of sun exposed non-MCC tumors [12 seborrheic keratoses (SK), 11 basal cell carcinomas (BCC) and 10 lentigo maligna melanomas (LMM)]. All tumors were analyzed for presence of MCPyV-DNA by polymerase chain reaction (PCR) and Southern-Blot hybridization of PCR products.ResultsMCPyV sequences were detected in 21 MCC samples (64%) and in 2 non-MCC tumors of sun exposed skin (6%; both SK-patients). Neither the tissue samples from BCC nor LMM proved positive for MCPyV sequences.ConclusionWe were able to confirm prior data on prevalence of MCPyV-DNA in MCC. Furthermore, a female predominance of MCPyV-positive MCC-patients was detected. There was no relevant association of MCPyV with SK, BCC and LMM. Speculative, prevalence of MCPyV in an age- and sex-matched non-MCC population could average up to 6%.Andres C, Belloni B, Puchta U, Sander CA, Flaig MJ. Is Merkel cell polyomavirus also prevalent in non-Merkel cell carcinoma (MCC) tumors of sun exposed skin? A study of 66 patients.