Modulating Responses of Toehold Switches by an Inhibitory Hairpin
Modulating Responses of Toehold Switches by an Inhibitory Hairpin
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DOI：
10.1021/acssynbio.8b00488
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发表时间：
2019-03-01
影响因子：
4.7
通讯作者：
Moon, Tae Seok
中科院分区：
文献类型：
--
作者：
Kim, Soo-Jung;Leong, Matthew;Moon, Tae Seok
支点开关由顺式抑制开关RNA发夹和反式作用触发RNA组成。触发RNA与开关发夹的未配对立足点序列的结合允许分支迁移过程，暴露起始密码子和核糖体结合位点用于翻译起始。在这项工作中，我们表明，可以通过引入抑制发夹，缩短未配对的立足点区域的长度调制的立足点开关的响应。首先，我们研究了立足点区域长度对输出基因表达的影响，并表明当发夹间距较短时，与抑制性发夹结合的第二触发RNA对于输出基因激活是必需的。第二，表观希尔系数一般被发现增加与减少发夹到发夹间距或增加发夹数量。这项工作扩大了实用的支点开关提供了一种新的方式来调节他们的反应。
The toehold switch consists of a cis-repressing switch RNA hairpin and a trans-acting trigger RNA. The binding of the trigger RNA to an unpaired toehold sequence of the switch hairpin allows for a branch migration process, exposing the start codon and ribosome binding site for translation initiation. In this work, we demonstrate that responses of toehold switches can be modulated by introducing an inhibitory hairpin that shortens the length of the unpaired toehold region. First, we investigated the effect of the toehold region length on output gene expression and showed that the second trigger RNA, which binds to the inhibitory hairpin, is necessary for output gene activation when the hairpin-to-hairpin spacing is short. Second, the apparent Hill coefficient was found generally to increase with decreasing hairpin-to-hairpin spacing or increasing hairpin number. This work expands the utility of toehold switches by providing a new way to modulate their response.