Peanut genes encoding tetrapyrrole biosynthetic enzymes, AhHEMA1 and AhFC1, alleviating the salt stress in transgenic tobacco
Peanut genes encoding tetrapyrrole biosynthetic enzymes, AhHEMA1 and AhFC1, alleviating the salt stress in transgenic tobacco
复制标题
编码四吡咯生物合成酶AhHEMA1和AhFC1的花生基因减轻转基因烟草的盐胁迫
DOI：
10.1016/j.plaphy.2019.01.027
复制
发表时间：
2019
影响因子：
6.5
通讯作者：
Li Xinguo
中科院分区：
文献类型：
--
作者：
Yang Sha;Zhao Luying;Yan Jianmei;Zhang Jialei;Guo Feng;Geng Yun;Wang Quan;Yang Fangyuan;Wan Shubo;Li Xinguo
谷氨酰-tRNA 还原酶 1 (HEMA1) 和亚铁螯合酶 1 (FC1) 均在四吡咯生物合成途径中响应盐胁迫而表达。通过 RT-PCR 分离花生 (ArachishypogaeaL.) HEMA1 和 FC1。这两个基因编码的氨基酸序列与其他植物物种的氨基酸序列表现出高度相似性。使用拟南芥叶肉原生质体验证了 AhFC1 融合蛋白在叶绿体中的功能。使用正义和野生型（WT）烟草来进一步研究AhHEMA1和AhFC1的生理效应。与WT相比，盐胁迫下AhFC1过表达植株的血红素含量和发芽率较高。同时，过表达AhHEMA1还导致ALA和叶绿素含量升高，以及盐胁迫下的多种生理变化，如超氧化物歧化酶（SOD）和抗坏血酸过氧化物酶（APX）活性升高、活性氧（ROS）含量降低和膜损伤轻微等。另外，盐胁迫下AhFC1过表达植株的CAT、POD和APX活性显着高于WT株系，但SOD活性在WT植株和转基因植株之间没有表现出显着差异。这些结果表明，花生可以通过改善四吡咯生物合成来增强对盐胁迫的抵抗力。
Glutamyl-tRNA reductase1 (HEMA1) and ferrochelatase1 (FC1) are both expressed in response to salt stress in the biosynthetic pathway of tetrapyrroles. Peanut (ArachishypogaeaL.) HEMA1 and FC1 were isolated by RT-PCR. The amino acid sequence encoded by the two genes showed high similarity with that in other plant species. The AhFC1 fusion protein was verified to function in chloroplast using Arabidopsis mesophyll protoplast. Sense and wild-type (WT) tobaccos were used to further study the physiological effects ofAhHEMA1and AhFC1. Compared with WT, the Heme contents and germination rate were higher in AhFC1 overexpressing plants under salt stress. Meanwhile, overexpressingAhHEMA1also led to higher ALA and chlorophyll contents and multiple physiological changes under salt stress, such as higher activities of superoxide dismutase (SOD) and ascorbate peroxidase (APX), lower contents of reactive oxygen species (ROS) and slighter membrane damage. In addition, the activities of CAT, POD and APX in theAhFC1overexpressing plants were significantly higher than that in WT lines under salt stress, but the activity of SOD between the WT plants and the transgenic plants did not exhibit significant differences. These results suggested that, peanut can enhance resistance to salt stress by improving the biosynthesis of tetrapyrrole biosynthetic.