Lipid and Carotenoid Production by Rhodotorula glutinis with a Combined Cultivation Mode of Nitrogen, Sulfur, and Aluminium Stress
Lipid and Carotenoid Production by Rhodotorula glutinis with a Combined Cultivation Mode of Nitrogen, Sulfur, and Aluminium Stress
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DOI：
10.3390/app9122444
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发表时间：
2019-06-02
影响因子：
2.7
通讯作者：
Bao, Yongming
中科院分区：
文献类型：
--
作者：
Elfeky, Nora;Elmahmoudy, Mostafa;Bao, Yongming
托苯二烯是一种很有前途的粉红色色素，仅由酵母和真菌生产，由于产量低，其生产仍处于发展阶段。因此，本研究的重点是利用摇瓶发酵法最大限度地提高粘红酵母的托苯二烯产量。在60 g/L葡萄糖条件下，研究了不同氮源、C/N和C/S比对粘滞红霉产脂和类胡萝卜素的影响。当蛋白胨作为氮源时，荧光显微镜观察到最大的细胞充满金色荧光脂质体。在C/N 146和C/S 120条件下，以硫酸铵为氮源，托苯二烯含量为62%，总色素（0.947 mg/L）和类胡萝卜素相对产率（Car-RP）最高（89.04 μ g/g）。在高C/N条件下，无论C/S比如何，3 d后类胡萝卜素合成率下降，而脂质合成率持续上升至第6 d。有趣的是，在优化的培养基中添加0.7 mM Al-2(SO4)(3)后，总色素和Car-RP （2.2 mg/L和212.9 μ g/g）急剧增加，产生约2.16 mg/L的甲苯（98%），脂质产量下降约50%。本文首次报道了Al-2(SO4)(3)在脂肪生成条件下促进多苯二烯生成的作用，可作为一种潜在的多苯二烯生成工具。
Torulene is a promising pink pigment, produced only by yeasts and fungi, and its production is still in a developing stage due to the low production rate. Accordingly, this study focuses on maximizing torulene production by Rhodotorula glutinis using shaken flask fermentation. The effect of different nitrogen sources, and C/N and C/S ratios on lipid and carotenoid production by R. glutinis was studied using 60 g/L glucose. The largest cells filled with golden fluorescence lipid bodies were observed using fluorescence microscopy when peptone was used as a nitrogen source. The highest total pigment (0.947 mg/L) and carotenoid relative productivity (Car-RP) (89.04 mu g/g) were obtained at C/N 146 and C/S 120, and with ammonium sulfate as a nitrogen source, with 62% torulene domination using High Performance Liquid Chromatography (HPLC) for identification. Under a high C/N ratio, regardless of the C/S ratio, the carotenoid synthesis rate decreased after three days while the lipid synthesis rate kept increasing to the sixth day. Interestingly, after adding 0.7 mM Al-2(SO4)(3) to the optimized medium, the total pigment and Car-RP (2.2 mg/L and 212.9 mu g/g) sharply increased, producing around 2.16 mg/L torulene (98%) with around 50% decrease in lipid yield. This is the first report on the role of Al-2(SO4)(3) for enhancing torulene production under lipogenesis condition, which could be used as a potential tool for torulene production.