LIP‐model‐based three‐dimensional reconstruction and visualization of HIV‐infected entire cells

LIP‐model‐based three‐dimensional reconstruction and visualization of HIV‐infected entire cells
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DOI:
10.1111/j.1365-2818.1994.tb04321.x
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发表时间:
1994-04
影响因子:
2
通讯作者:
P. Gremillet;Michel Jourlin;J. Pinoli
P. Gremillet;Michel Jourlin;J. Pinoli
中科院分区:
工程技术4区
文献类型:
--
作者:
P. Gremillet;Michel Jourlin;J. Pinoli

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本文提出了一种从获取到可视化的细胞切片三维重建的全局解决方案。提出了用于正确处理几何截面失真的原始技术,并应用了基于对数图像处理(LIP)模型的新解释,以便在缺失几何截面的情况下创建归一化灰度截面。最后,提出了一种描述重构细胞包络的三角形网格生成方法,以及融合图像合成和灰度信息的可视化方法。该产品允许用户以任何所需的方向探索重建的细胞块,方法是将块内用户定义的灰色部分混合到细胞包膜的合成视图中。
This paper presents a global solution from acquisition to visualization for the three‐dimensional reconstruction of cell sections. Original techniques are proposed for the correct handling of the geometrical section distortions, and a new interpretation based on the logarithmic image processing (LIP) model is applied in order to create normalized grey‐level sections where these are missing. Finally, a new method for generating a mesh of triangles to describe the envelope of the reconstructed cell is proposed, as well as a visualization mixing image synthesis and grey‐level information. The product allows the user to explore the reconstructed cellular block in any desired direction, by showing user‐defined grey‐level sections inside the block mixed to a synthetic view of the cell envelope.